中国物理B ›› 2018, Vol. 27 ›› Issue (2): 27803-027803.doi: 10.1088/1674-1056/27/2/027803
• CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES • 上一篇 下一篇
Chao-Fan Zhu(朱超凡), Xue Sha(沙雪), Xue-Ying Chu(楚学影), Jin-Hua Li(李金华), Ming-Ze Xu(徐铭泽), Fang-Jun Jin(金芳军), Zhi-Kun Xu(徐志堃)
Chao-Fan Zhu(朱超凡)1, Xue Sha(沙雪)1, Xue-Ying Chu(楚学影)1, Jin-Hua Li(李金华)1, Ming-Ze Xu(徐铭泽)1, Fang-Jun Jin(金芳军)1, Zhi-Kun Xu(徐志堃)2
摘要: Biofunctional europium (Ⅲ)-doped ZnS (ZnS:Eu) nanocrystals are prepared by a sol-gel method. The characteristic luminescence of ZnS:Eu is used as a probe signal to realize sensitive immunoassay. The luminescence intensity of the Eu3+ in the ZnS matrix shows strong concentration dependence, and the optimal doping concentration is 4%. However, the emission wavelengths of the ZnS:Eu nanocrystals are not dependent on doping concentration nor the temperature (from 100 K to 300 K). Our results show that these features allow for reliable immunoassay. Human immunoglobulin, used as a target analyte, is captured by antibody modified ZnS:Eu probe and is finally enriched on gold substrate for detection. High specificity of the assay is demonstrated by control experiments. The linear detection range is 10 nM-800 nM, and the detection limit is about 9.6 nM.
中图分类号: (Photoluminescence, properties and materials)