In situ calibrating optical tweezers with sinusoidal-wave drag force method

doi:10.1088/1674-1056/24/11/118703

中国物理B ›› 2015, Vol. 24 ›› Issue (11): 118703-118703.doi: 10.1088/1674-1056/24/11/118703

• INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY • 上一篇下一篇

In situ calibrating optical tweezers with sinusoidal-wave drag force method

李迪^a, 周金华^a, 呼新尧^a, 钟敏成^a, 龚雷^a, 王自强^a, 王浩威^a, 李银妹^{a b}

a Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei 230026, China;
b Hefei National Laboratory for Physical Sciences at the Microscale, Hefei 230026, China

收稿日期:2015-04-30 修回日期:2015-06-15 出版日期:2015-11-05 发布日期:2015-11-05
通讯作者: Li Yin-Mei E-mail:liyinmei@ustc.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant Nos. 11302220, 11374292, and 31100555) and the National Basic Research Program of China (Grant No. 2011CB910402).

In situ calibrating optical tweezers with sinusoidal-wave drag force method

Li Di (李迪)^a, Zhou Jin-Hua (周金华)^a, Hu Xin-Yao (呼新尧)^a, Zhong Min-Cheng (钟敏成)^a, Gong Lei (龚雷)^a, Wang Zi-Qiang (王自强)^a, Wang Hao-Wei (王浩威)^a, Li Yin-Mei (李银妹)^{a b}

a Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei 230026, China;
b Hefei National Laboratory for Physical Sciences at the Microscale, Hefei 230026, China

Received:2015-04-30 Revised:2015-06-15 Online:2015-11-05 Published:2015-11-05
Contact: Li Yin-Mei E-mail:liyinmei@ustc.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant Nos. 11302220, 11374292, and 31100555) and the National Basic Research Program of China (Grant No. 2011CB910402).

摘要/Abstract

摘要： We introduce a corrected sinusoidal-wave drag force method (SDFM) into optical tweezers to calibrate the trapping stiffness of the optical trap and conversion factor (CF) of photodetectors. First, the theoretical analysis and experimental result demonstrate that the correction of SDFM is necessary, especially the error of no correction is up to 11.25% for a bead of 5 μm in diameter. Second, the simulation results demonstrate that the SDFM has a better performance in the calibration of optical tweezers than the triangular-wave drag force method (TDFM) and power spectrum density method (PSDM) at the same signal-to-noise ratio or trapping stiffness. Third, in experiments, the experimental standard deviations of calibration of trapping stiffness and CF with the SDFM are about less than 50% of TDFM and PSDM especially at low laser power. Finally, the experiments of stretching DNA verify that the in situ calibration with the SDFM improves the measurement stability and accuracy.

关键词: optical tweezers, trapping stiffness calibration, conversion factor, drag force method

Abstract: We introduce a corrected sinusoidal-wave drag force method (SDFM) into optical tweezers to calibrate the trapping stiffness of the optical trap and conversion factor (CF) of photodetectors. First, the theoretical analysis and experimental result demonstrate that the correction of SDFM is necessary, especially the error of no correction is up to 11.25% for a bead of 5 μm in diameter. Second, the simulation results demonstrate that the SDFM has a better performance in the calibration of optical tweezers than the triangular-wave drag force method (TDFM) and power spectrum density method (PSDM) at the same signal-to-noise ratio or trapping stiffness. Third, in experiments, the experimental standard deviations of calibration of trapping stiffness and CF with the SDFM are about less than 50% of TDFM and PSDM especially at low laser power. Finally, the experiments of stretching DNA verify that the in situ calibration with the SDFM improves the measurement stability and accuracy.

Key words: optical tweezers, trapping stiffness calibration, conversion factor, drag force method

中图分类号: (Optical trapping)

87.80.Cc

42.50.Wk (Mechanical effects of light on material media, microstructures and particles) 87.14.gk (DNA)

李迪, 周金华, 呼新尧, 钟敏成, 龚雷, 王自强, 王浩威, 李银妹. In situ calibrating optical tweezers with sinusoidal-wave drag force method[J]. 中国物理B, 2015, 24(11): 118703-118703.

Li Di (李迪), Zhou Jin-Hua (周金华), Hu Xin-Yao (呼新尧), Zhong Min-Cheng (钟敏成), Gong Lei (龚雷), Wang Zi-Qiang (王自强), Wang Hao-Wei (王浩威), Li Yin-Mei (李银妹). In situ calibrating optical tweezers with sinusoidal-wave drag force method[J]. Chin. Phys. B, 2015, 24(11): 118703-118703.

参考文献

[1] Moffitt J R, Chemla Y R, Smith S B and Bustamante C;2008 Recent Advances in Optical Tweezers (Palo Alto: Annual Reviews) p. 205
[2] Guo H, Qu E, Xu C, Li Z, Cheng B and Zhang D 2007 Physics 36 476
[3] Xu C H, Guo H L, Qu E, Li Z L, Yuan M, Cheng B Y and Zhang D Z 2007 Chin. Phys. Lett. 24 2714
[4] Xia P, Zhou J H, Song X Y, Wu B, Liu X, Li D, Zhang S Y, Wang Z K, Yu H J, Ward T, Zhang J C, Li Y M, Wang X N, Chen Y, Guo Z and Yao X B;2014 J. Mol. Cell Biol. 6 240
[5] Zhong M C, Wei X B, Zhou J H, Wang Z Q and Li Y M;2013 Nat. Commun. 4 1768
[6] Zhong M C, Gong L, Zhou J H, Wang Z Q and Li Y M;2013 Opt. Lett. 38 5134
[7] Zhong M C, Gong L, Li D, Zhou J H, Wang Z Q and Li Y M;2014 Appl. Phys. Lett. 105 181112
[8] Bishop A I, Nieminen T A, Heckenberg N R and Rubinsztein-Dunlop H;2004 Phys. Rev. Lett. 92 198104
[9] Shen G, Xue G, Cai J, Zou G, Li Y and Zhang Q;2013 Soft Matter 9 2512
[10] Xue G, Chen K, Shen G, Wang Z, Zhang Q, Cai J and Li Y;2013 Colloid. Surf. A 436 1007
[11] Ling L, Guo H L, Huang L, Qu E, Li Z L and Li Z Y;2012 Chin. Phys. Lett. 29
[12] Berg-Sorensen K and Flyvbjerg H;2004 Rev. Sci. Instrum. 75 594
[13] Wang Z Q, Zhou J H, Zhong M C, Li D and Li Y M;2014 Opt. Express 22 16956
[14] Li X C and Sun X D 2010 Chin. Phys. B 19 119401
[15] Visscher K, Gross S P and Block S M 1996 IEEE J. Sel. Top. Quantum Electron. 2 1066
[16] Gittes F and Schmidt C F;1998 Opt. Lett. 23 7
[17] Hajizadeh F, Mousavi S M, Khaksar Z S and Reihani S N S;2014 J. Opt. 16 105706
[18] Svoboda K, Schmidt C F, Schnapp B J and Block S M;1993 Nature 365 721
[19] Ghislain L P, Switz N A and Webb W W;1994 Rev. Sci. Instrum. 65 2762
[20] Capitanio M, Romano G, Ballerini R, Giuntini M, Pavone F, Dunlap D and Finzi L;2002 Rev. Sci. Instrum. 73 1687
[21] Svoboda K and Block S M;1994 Annu. Rev. Biophys. Biomol. Struct. 23 247
[22] Wang M D, Yin H, Landick R, Gelles J and Block S M;1997 Biophys. J. 72 1335
[23] Tolić-Norrelykke S F, Schaffer E, Howard J, Pavone F S, Julicher F and Flyvbjerg H;2006 Rev. Sci. Instrum. 77 103101
[24] Valentine M T, Dewalt L E and Ou-Yang H D;1996 J. Phys.: Condens. Matter 8 9477
[25] Wei M T, Zaorski A, Yalcin H C, Wang J, Hallow M, Ghadiali S N, Chiou A and Ou-Yang H D;2008 Opt. Express 16 8594
[26] Bechhoefer J and Wilson S;2002 Am. J. Phys. 70 393
[27] Wang Z Q, Zhong M C, Zhou J H and Li Y M;2013 Acta Phys. Sin. 62 188701 (in Chinese)
[28] Czerwinski F, Richardson A C and Oddershede L B;2009 Opt. Express 17 13255
[29] Allersma M W, Gittes F, Decastro M J, Stewart R J and Schmidt C F;1998 Biophys. J. 74 1074
[30] Pralle A, Prummer M, Florin E L, Stelzer E H K and Horber J K H;1999 Microsc. Res. Technol. 44 378
[31] Kheifets S, Simha A, Melin K, Li T C and Raizen M G;2014 Science 343 1493
[32] Gong Z, Wang Z, Li Y M, Lou L R and Xu S H;2007 Opt. Commun. 273 37
[33] Salomo M, Kegler K, Gutsche C, Struhalla M, Reinmuth J, Skokow W, Hahn U and Kremer F;2006 Colloid. Polym. Sci. 284 1325

In situ calibrating optical tweezers with sinusoidal-wave drag force method

In situ calibrating optical tweezers with sinusoidal-wave drag force method

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