中国物理B ›› 2008, Vol. 17 ›› Issue (6): 2288-2291.doi: 10.1088/1674-1056/17/6/059

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Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents

李巍1, 梁汝强1, 周岳亮1, 阮康成1, 陆珩2, 金奎娟2, 杨国桢2, 张洪艳3   

  1. (1)Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China; (2)Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100190, China; (3)Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100190, China;Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering, Technical Institute of Ph
  • 收稿日期:2007-10-15 修回日期:2007-10-30 出版日期:2008-06-20 发布日期:2008-06-20
  • 基金资助:
    Project supported by the Chinese Academy of Sciences for Key Topics in Innovation Engineering (Grant No L3FX051W).

Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents

Zhang Hong-Yan(张洪艳)a)b)†, Lu Heng(陆珩)a), Li Wei(李巍)c), Liang Ru-Qiang(梁汝强)c), Jin Kui-Juan(金奎娟)a), Zhou Yue-Liang(周岳亮)a), Ruan Kang-Cheng (阮康成)c), and Yang Guo-Zhen(杨国桢)a)   

  1. a Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100190, China; b Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China; c Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
  • Received:2007-10-15 Revised:2007-10-30 Online:2008-06-20 Published:2008-06-20
  • Supported by:
    Project supported by the Chinese Academy of Sciences for Key Topics in Innovation Engineering (Grant No L3FX051W).

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摘要： This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI--RD) analysis without any labelling agents. The OI--RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.

关键词: detection, OI--RD, protein, microarray

Abstract: This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI--RD) analysis without any labelling agents. The OI--RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.

Key words: detection, OI--RD, protein, microarray

中图分类号:  (Proteins)

  • 87.14.E-
87.15.R- (Reactions and kinetics) 87.80.-y (Biophysical techniques (research methods))