中国物理B ›› 2005, Vol. 14 ›› Issue (4): 744-752.doi: 10.1088/1009-1963/14/4/018
谢平, 窦硕星, 王鹏业
Xie Ping (谢平), Dou Shuo-Xing (窦硕星), Wang Peng-Ye (王鹏业)
摘要: Myosin V and myosin VI are two classes of two-headed molecular motors of the myosin superfamily that move processively along helical actin filaments in opposite directions. Here we present a hand-over-hand model for their processive movements. In the model, the moving direction of a dimeric molecular motor is automatically determined by the relative orientation between its two heads at free state and its head's binding orientation on track filament. This determines that myosin V moves toward the barbed end and myosin VI moves toward the pointed end of actin. During the moving period in one step, one head remains bound to actin for myosin V whereas two heads are detached for myosin VI: the moving manner is determined by the length of neck domain. This naturally explains the similar dynamic behaviours but opposite moving directions of myosin VI and mutant myosin V (the neck of which is truncated to only one-sixth of the native length). Because of different moving manners, myosin VI and mutant myosin V exhibit significantly broader step-size distribution than native myosin V. However,all the three motors give the same mean step size of $\sim $36nm (the pseudo-repeat of actin helix). All these theoretical results are in agreement with previous experimental ones.
中图分类号: (Structure of biomolecules)