中国物理B ›› 2002, Vol. 11 ›› Issue (8): 776-784.doi: 10.1088/1009-1963/11/8/305

• GENERAL • 上一篇    下一篇

Lipid membrane: inelastic deformation of surface structure by an atomic force microscope

张静, 孙润广   

  1. Department of Food Engineering, College of Physics and Information Technology and Shaanxi Normal University 710062 Xi'an, China
  • 收稿日期:2001-10-21 修回日期:2002-03-21 出版日期:2005-06-12 发布日期:2005-06-12
  • 基金资助:
    Project supported by the National Natural Science Foundation of China (Grant No 29672024) and the Foundation for University Key Teachers by the Ministry of Education, China (Grant No 2000-65)

Lipid membrane: inelastic deformation of surface structure by an atomic force microscope

Zhang Jing (张静), Sun Run-Guang (孙润广)   

  1. Department of Food Engineering, College of Physics and Information Technology and Shaanxi Normal University 710062 Xi'an, China
  • Received:2001-10-21 Revised:2002-03-21 Online:2005-06-12 Published:2005-06-12
  • Supported by:
    Project supported by the National Natural Science Foundation of China (Grant No 29672024) and the Foundation for University Key Teachers by the Ministry of Education, China (Grant No 2000-65)

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摘要： The stability of the 1,2-Dioleoyl-sn-Glycero-3-[phospho-rac-1-Glycerol-Na] liposome in the liquid crystalline state have been investigated using an atomic force microscope (AFM). We have observed the inelastic deformation of the sample surface. The AFM tip causes persistent deformation of the surface of the lipid membrane, in which some of the lipid molecules are eventually pushed or dragged by the AFM tip. The experiment shows how the surface structure of the lipid membrane can be created by the interaction between the AFM tip and lipid membrane. When the operating force exceeds 10-8 N, it leads to large deformations of the surface. A square region of about 1×1μm2 is created by the scanning probe on the surface. When the operating force is between 10-11N and 10-8N, it can image the topography of the surface of the lipid membrane. The stability of the sample is related to the concentration of the medium in which the sample is prepared.

Abstract: The stability of the 1,2-Dioleoyl-sn-Glycero-3-[phospho-rac-1-Glycerol-Na] liposome in the liquid crystalline state have been investigated using an atomic force microscope (AFM). We have observed the inelastic deformation of the sample surface. The AFM tip causes persistent deformation of the surface of the lipid membrane, in which some of the lipid molecules are eventually pushed or dragged by the AFM tip. The experiment shows how the surface structure of the lipid membrane can be created by the interaction between the AFM tip and lipid membrane. When the operating force exceeds 10-8 N, it leads to large deformations of the surface. A square region of about 1×1μm2 is created by the scanning probe on the surface. When the operating force is between 10-11N and 10-8N, it can image the topography of the surface of the lipid membrane. The stability of the sample is related to the concentration of the medium in which the sample is prepared.

Key words: atomic force microscope, lipid bilayers, liquid crystalline state

中图分类号:  (Lipids)

  • 87.14.Cc
62.10.+s (Mechanical properties of liquids)