中国物理B ›› 2025, Vol. 34 ›› Issue (7): 70704-070704.doi: 10.1088/1674-1056/adde38

所属专题： Featured Column — INSTRUMENTATION AND MEASUREMENT

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Spin-based magnetic detection of optically trapped single cell in microfluidic channel

Jun Yin(殷俊)1,2,3, Sanyou Chen(陈三友)1,2,3, Yihao Yan(燕一皓)1,4, Mengqi Wang(王孟祺)1,2, Ya Wang(王亚)1,2,4, Yiheng Lin(林毅恒)1,2,4, Qi Zhang(张琪)3,5,†, and Fazhan Shi(石发展)1,2,3,4,‡   

  1. 1 School of Physical Sciences, University of Science and Technology of China, Hefei 230026, China;
    2 Anhui Province Key Laboratory of Scientific Instrument Development and Application, University of Science and Technology of China, Hefei 230026, China;
    3 School of Biomedical Engineering and Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, China;
    4 Hefei National Laboratory, University of Science and Technology of China, Hefei 230088, China;
    5 Institute of Quantum Sensing, School of Physics, Institute of Fundamental and Transdisciplinary Research, Zhejiang Key Laboratory of R&D and Application of Cuttingedge Scientifc Instruments, State Key Laboratory of Ocean Sensing, Zhejiang University, Hangzhou 310027, China
  • 收稿日期:2025-04-08 修回日期:2025-05-29 接受日期:2025-05-29 出版日期:2025-06-18 发布日期:2025-07-03
  • 通讯作者: Qi Zhang, Fazhan Shi E-mail:zhq2011@ustc.edu.cn;fzshi@ustc.edu.cn
  • 基金资助:
    Project supported by the the National Key R&D Program of China (Grant Nos. 2019YFA0709300 and 2021YFB3202800), the National Natural Science Foundation of China (Grant Nos. T2125011 and 12174377), the Chinese Academy of Sciences (Grant No. YSBR-068), Innovation Program for Quantum Science and Technology (Grant Nos. 2021ZD0302200 and 2021ZD0303204), New Cornerstone Science Foundation through the XPLORER PRIZE, Science and Technology Department of Zhejiang Province (Grant No. 2025C01041), and the Fundamental Research Funds for the Central Universities (Grant No. 226-2024-00142).

Spin-based magnetic detection of optically trapped single cell in microfluidic channel

Jun Yin(殷俊)1,2,3, Sanyou Chen(陈三友)1,2,3, Yihao Yan(燕一皓)1,4, Mengqi Wang(王孟祺)1,2, Ya Wang(王亚)1,2,4, Yiheng Lin(林毅恒)1,2,4, Qi Zhang(张琪)3,5,†, and Fazhan Shi(石发展)1,2,3,4,‡   

  1. 1 School of Physical Sciences, University of Science and Technology of China, Hefei 230026, China;
    2 Anhui Province Key Laboratory of Scientific Instrument Development and Application, University of Science and Technology of China, Hefei 230026, China;
    3 School of Biomedical Engineering and Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, China;
    4 Hefei National Laboratory, University of Science and Technology of China, Hefei 230088, China;
    5 Institute of Quantum Sensing, School of Physics, Institute of Fundamental and Transdisciplinary Research, Zhejiang Key Laboratory of R&D and Application of Cuttingedge Scientifc Instruments, State Key Laboratory of Ocean Sensing, Zhejiang University, Hangzhou 310027, China
  • Received:2025-04-08 Revised:2025-05-29 Accepted:2025-05-29 Online:2025-06-18 Published:2025-07-03
  • Contact: Qi Zhang, Fazhan Shi E-mail:zhq2011@ustc.edu.cn;fzshi@ustc.edu.cn
  • Supported by:
    Project supported by the the National Key R&D Program of China (Grant Nos. 2019YFA0709300 and 2021YFB3202800), the National Natural Science Foundation of China (Grant Nos. T2125011 and 12174377), the Chinese Academy of Sciences (Grant No. YSBR-068), Innovation Program for Quantum Science and Technology (Grant Nos. 2021ZD0302200 and 2021ZD0303204), New Cornerstone Science Foundation through the XPLORER PRIZE, Science and Technology Department of Zhejiang Province (Grant No. 2025C01041), and the Fundamental Research Funds for the Central Universities (Grant No. 226-2024-00142).

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摘要： Combining optical tweezers with fluorescence microscopy is a powerful tool for single-cell analysis, playing a pivotal role in disease diagnosis, cell sorting, and the investigation of cellular dynamics. However, fluorescence detection faces challenges such as blinking, photobleaching and autofluorescence in biotissues. To address these limitations, we developed a magnetic detection strategy by integrating quantum magnetometry using nitrogen-vacancy centers into optical tweezers, demonstrating precise trapping and manipulation of individual cells in microfluidic environment. We detected a magnetic signal of 89 μT from a single cell labeled with magnetic nanoparticles, compared to a noise floor of 3.9 μT observed in unlabeled cells. This platform provides a promising approach for high-precision single-cell analysis and holds significant potential for probing cellular activities within biological microenvironments.

关键词: nitrogen-vacancy center, magnetic sensing, optical tweezers, single-cell analysis

Abstract: Combining optical tweezers with fluorescence microscopy is a powerful tool for single-cell analysis, playing a pivotal role in disease diagnosis, cell sorting, and the investigation of cellular dynamics. However, fluorescence detection faces challenges such as blinking, photobleaching and autofluorescence in biotissues. To address these limitations, we developed a magnetic detection strategy by integrating quantum magnetometry using nitrogen-vacancy centers into optical tweezers, demonstrating precise trapping and manipulation of individual cells in microfluidic environment. We detected a magnetic signal of 89 μT from a single cell labeled with magnetic nanoparticles, compared to a noise floor of 3.9 μT observed in unlabeled cells. This platform provides a promising approach for high-precision single-cell analysis and holds significant potential for probing cellular activities within biological microenvironments.

Key words: nitrogen-vacancy center, magnetic sensing, optical tweezers, single-cell analysis

中图分类号:  (Magnetometers for magnetic field measurements)

  • 07.55.Ge
76.30.Mi (Color centers and other defects) 87.80.-y (Biophysical techniques (research methods)) 87.80.Cc (Optical trapping)