Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

doi:10.1088/1674-1056/ad334c

中国物理B ›› 2024, Vol. 33 ›› Issue (5): 58706-058706.doi: 10.1088/1674-1056/ad334c

Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

Jingru Yao(姚静如)^1,†, Guoqiang Li(李国强)^2,†,‡, Xiyao Yao(姚喜耀)¹, Lianjie Zhou(周连杰)¹, Zhikai Ye(叶志凯)³, Yanping Liu(刘艳平)⁴, Dongtian Zheng(郑栋天)¹, Ting Tang(唐婷)², Kena Song(宋克纳)⁵, Guo Chen(陈果)¹, and Liyu Liu(刘雳宇)^1,§

1 Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, College of Physics, Chongqing University, Chongqing 401331, China;
2 Chongqing Key Laboratory for Resource Utilization of Heavy Metal Wastewater, Aquatic Ecosystems in the Three Gorges Reservoir Region of Chongqing Observation and Research Station, Chongqing University of Arts and Sciences, Yongchuan 402160, China;
3 Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325000, China;
4 Department of Biomedical Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China;
5 College of Medical Technology and Engineering, Henan University of Science and Technology, Henan 471023, China}

收稿日期:2024-02-02 修回日期:2024-03-02 接受日期:2024-03-13 出版日期:2024-05-20 发布日期:2024-05-20
通讯作者: Guoqiang Li, Liyu Liu E-mail:guoqiangli1989@126.com;lyliu@cqu.edu.cn
基金资助:
We gratefully acknowledge support from the National Natural Science Foundation of China (Grant Nos. 11974066, 12174041, 12104134, T2350007, and 12347178) and the Fundamental and Advanced Research Program of Chongqing (Grant No. cstc2019jcyj-msxmX0477), and the Natural Science Foundation of Chongqing (Grant No. CSTB2022NSCQMSX1260), the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJQN202301333), the Scientific Research Fund of Chongqing University of Arts and Sciences (Grant Nos. R2023HH03 and P2022HH05), College Students’ Innovation and Entrepreneurship Training Program of Chongqing Municipal (Grant No. S202310642002).

Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

1 Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, College of Physics, Chongqing University, Chongqing 401331, China;
2 Chongqing Key Laboratory for Resource Utilization of Heavy Metal Wastewater, Aquatic Ecosystems in the Three Gorges Reservoir Region of Chongqing Observation and Research Station, Chongqing University of Arts and Sciences, Yongchuan 402160, China;
3 Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325000, China;
4 Department of Biomedical Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China;
5 College of Medical Technology and Engineering, Henan University of Science and Technology, Henan 471023, China}

Received:2024-02-02 Revised:2024-03-02 Accepted:2024-03-13 Online:2024-05-20 Published:2024-05-20
Contact: Guoqiang Li, Liyu Liu E-mail:guoqiangli1989@126.com;lyliu@cqu.edu.cn
Supported by:
We gratefully acknowledge support from the National Natural Science Foundation of China (Grant Nos. 11974066, 12174041, 12104134, T2350007, and 12347178) and the Fundamental and Advanced Research Program of Chongqing (Grant No. cstc2019jcyj-msxmX0477), and the Natural Science Foundation of Chongqing (Grant No. CSTB2022NSCQMSX1260), the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJQN202301333), the Scientific Research Fund of Chongqing University of Arts and Sciences (Grant Nos. R2023HH03 and P2022HH05), College Students’ Innovation and Entrepreneurship Training Program of Chongqing Municipal (Grant No. S202310642002).

1. 2024-058706-SI.pdf(681KB)

摘要/Abstract

摘要： Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment, playing a crucial role in pathological processes such as tumor invasion and metastasis. However, conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell migration. Fortunately, the further development of microfluidic technology has provided solutions for the study of such questions. We utilize microfluidic chip to build a random collagen fiber microenvironment (RFM) model and an oriented collagen fiber microenvironment (OFM) model that resemble early stage and late stage breast cancer microenvironments, respectively. By combining cell culture, biochemical concentration gradient construction, and microscopic imaging techniques, we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP cells. The results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model, with significant differences observed. Furthermore, we establish concentration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model, with stronger inhibition on the high concentration side compared to the low concentration side. However, the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is weak. These findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is diminished. The RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development, but also serve as a tool for the initial measurement of drug screening.

关键词: microfluidic chip, random collagen fiber microenvironment (RFM), oriented collagen fiber microenvironment (OFM), cancer cell migration

Abstract: Increasing data indicate that cancer cell migration is regulated by extracellular matrixes and their surrounding biochemical microenvironment, playing a crucial role in pathological processes such as tumor invasion and metastasis. However, conventional two-dimensional cell culture and animal models have limitations in studying the influence of tumor microenvironment on cancer cell migration. Fortunately, the further development of microfluidic technology has provided solutions for the study of such questions. We utilize microfluidic chip to build a random collagen fiber microenvironment (RFM) model and an oriented collagen fiber microenvironment (OFM) model that resemble early stage and late stage breast cancer microenvironments, respectively. By combining cell culture, biochemical concentration gradient construction, and microscopic imaging techniques, we investigate the impact of different collagen fiber biochemical microenvironments on the migration of breast cancer MDA-MB-231-RFP cells. The results show that MDA-MB-231-RFP cells migrate further in the OFM model compared to the RFM model, with significant differences observed. Furthermore, we establish concentration gradients of the anticancer drug paclitaxel in both the RFM and OFM models and find that paclitaxel significantly inhibits the migration of MDA-MB-231-RFP cells in the RFM model, with stronger inhibition on the high concentration side compared to the low concentration side. However, the inhibitory effect of paclitaxel on the migration of MDA-MB-231-RFP cells in the OFM model is weak. These findings suggest that the oriented collagen fiber microenvironment resembling the late-stage tumor microenvironment is more favorable for cancer cell migration and that the effectiveness of anticancer drugs is diminished. The RFM and OFM models constructed in this study not only provide a platform for studying the mechanism of cancer development, but also serve as a tool for the initial measurement of drug screening.

Key words: microfluidic chip, random collagen fiber microenvironment (RFM), oriented collagen fiber microenvironment (OFM), cancer cell migration

中图分类号: (Cells on a chip)

87.85.dh

87.80.-y (Biophysical techniques (research methods)) 87.18.Gh (Cell-cell communication; collective behavior of motile cells) 87.50.cf (Biophysical mechanisms of interaction)

Jingru Yao(姚静如), Guoqiang Li(李国强), Xiyao Yao(姚喜耀), Lianjie Zhou(周连杰), Zhikai Ye(叶志凯), Yanping Liu(刘艳平), Dongtian Zheng(郑栋天), Ting Tang(唐婷), Kena Song(宋克纳), Guo Chen(陈果), and Liyu Liu(刘雳宇). Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments[J]. 中国物理B, 2024, 33(5): 58706-058706.

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Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

Estimation of cancer cell migration in biomimetic random/oriented collagen fiber microenvironments

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