Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

doi:10.1088/1674-1056/19/4/049501

中国物理B ›› 2010, Vol. 19 ›› Issue (4): 49501-049501.doi: 10.1088/1674-1056/19/4/049501

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Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

卓双木¹, 喻碧英¹, 庄正飞², 刘汉平², 郭周义², 邓小元²

(1)Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China; (2)MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510631, China

收稿日期:2009-01-02 修回日期:2009-10-10 出版日期:2010-04-15 发布日期:2010-04-15
基金资助:
Project supported by the National Natural Science Foundation of China (Grant No.~30470495), the Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Normal University.

Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

Zhuang Zheng-Fei(庄正飞)^a), Liu Han-Ping(刘汉平)^a), Guo Zhou-Yi(郭周义)^a), Zhuo Shuang-Mu(卓双木)^b), Yu Bi-Ying(喻碧英)^b), and Deng Xiao-Yuan(邓小元)^a)†

^a MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510631, China; ^b Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China

Received:2009-01-02 Revised:2009-10-10 Online:2010-04-15 Published:2010-04-15
Supported by:
Project supported by the National Natural Science Foundation of China (Grant No.~30470495), the Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Normal University.

摘要/Abstract

摘要： This paper first demonstrates second-harmonic generation (SHG) in the intact cell nucleus, which acts as an optical indicator of DNA malignancy in prostate glandular epithelial cells. Within a scanning region of $2.7~\mu$m$\times 2.7~\mu$m in cell nuclei, SHG signals produced from benign prostatic hyperplasia (BPH) and prostate carcinoma (PC) tissues (mouse model C57BL/6) have been investigated. Statistical analyses ($t$ test) of a total of 405 measurements (204 nuclei from BPH and 201 nuclei from PC) show that SHG signals from BPH and PC have a distinct difference ($p < 0.05)$, suggesting a potential optical method of revealing very early malignancy in prostate glandular epithelial cells based upon induced biochemical and/or biophysical modifications in DNA.

Abstract: This paper first demonstrates second-harmonic generation (SHG) in the intact cell nucleus, which acts as an optical indicator of DNA malignancy in prostate glandular epithelial cells. Within a scanning region of $2.7~\mu$m$\times 2.7~\mu$m in cell nuclei, SHG signals produced from benign prostatic hyperplasia (BPH) and prostate carcinoma (PC) tissues (mouse model C57BL/6) have been investigated. Statistical analyses ($t$ test) of a total of 405 measurements (204 nuclei from BPH and 201 nuclei from PC) show that SHG signals from BPH and PC have a distinct difference ($p < 0.05)$, suggesting a potential optical method of revealing very early malignancy in prostate glandular epithelial cells based upon induced biochemical and/or biophysical modifications in DNA.

Key words: nonlinear microscopy, multiharmonic generation, diagnostic applications of nonlinear optics

中图分类号: (Nucleic acids)

87.14.G-

42.65.Ky (Frequency conversion; harmonic generation, including higher-order harmonic generation) 87.19.X- (Diseases) 87.15.N- (Properties of solutions of macromolecules) 87.15.M- (Spectra of biomolecules)

庄正飞, 刘汉平, 郭周义, 卓双木, 喻碧英, 邓小元. Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells[J]. 中国物理B, 2010, 19(4): 49501-049501.

Zhuang Zheng-Fei(庄正飞), Liu Han-Ping(刘汉平), Guo Zhou-Yi(郭周义), Zhuo Shuang-Mu(卓双木), Yu Bi-Ying(喻碧英), and Deng Xiao-Yuan(邓小元). Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells[J]. Chin. Phys. B, 2010, 19(4): 49501-049501.

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Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

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