中国物理B ›› 2026, Vol. 35 ›› Issue (5): 54207-054207.doi: 10.1088/1674-1056/ae067f

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Highly sensitive, ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe

Yufang Chen(陈彧芳)1,†, Wenbo Zhao(赵文博)2, Jinxing Xiong(熊金鑫)2, Jiashu Zhang(张家树)1, Kai Zhang(张开)2, Jiale Xie(解嘉乐)2, Menghan Liu(刘梦涵)1, and Hongdan Wan(万洪丹)2,‡   

  1. 1 School of Network and Communication, Nanjing Vocational College of Information Technology, Nanjing 210023, China;
    2 Advanced Photonic Technology Laboratory, Nanjing University of Posts and Telecommunications, Nanjing 210023, China
  • 收稿日期:2025-08-01 修回日期:2025-09-03 接受日期:2025-09-15 发布日期:2026-05-11
  • 通讯作者: Yufang Chen, Hongdan Wan E-mail:chenyf@njcit.cn;hdwan@njupt.edu.cn
  • 基金资助:
    This work was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Grant Nos. 25KJB416001 and 22KJB510034), the National Natural Science Foundation of China (Grant Nos. 12174199 and 11704199), the Scientific Research Foundation for Highlevel Talents in Nanjing Vocational College of Information Technology (Grant No. YB202410), the China Postdoctoral Science Foundation (Grant No. 2021M701765), General Program of Natural Science Foundation of Jiangsu Province (Grant No. BK20221330), Jiangsu University ‘Blue Project’ Funding, Postgraduate Research & Practice Innovation Programs of Jiangsu Province, and Jiangsu Province Higher Education Teaching Reform Research Project (Grant No. 2025JGYB487).

Highly sensitive, ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe

Yufang Chen(陈彧芳)1,†, Wenbo Zhao(赵文博)2, Jinxing Xiong(熊金鑫)2, Jiashu Zhang(张家树)1, Kai Zhang(张开)2, Jiale Xie(解嘉乐)2, Menghan Liu(刘梦涵)1, and Hongdan Wan(万洪丹)2,‡   

  1. 1 School of Network and Communication, Nanjing Vocational College of Information Technology, Nanjing 210023, China;
    2 Advanced Photonic Technology Laboratory, Nanjing University of Posts and Telecommunications, Nanjing 210023, China
  • Received:2025-08-01 Revised:2025-09-03 Accepted:2025-09-15 Published:2026-05-11
  • Contact: Yufang Chen, Hongdan Wan E-mail:chenyf@njcit.cn;hdwan@njupt.edu.cn
  • Supported by:
    This work was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Grant Nos. 25KJB416001 and 22KJB510034), the National Natural Science Foundation of China (Grant Nos. 12174199 and 11704199), the Scientific Research Foundation for Highlevel Talents in Nanjing Vocational College of Information Technology (Grant No. YB202410), the China Postdoctoral Science Foundation (Grant No. 2021M701765), General Program of Natural Science Foundation of Jiangsu Province (Grant No. BK20221330), Jiangsu University ‘Blue Project’ Funding, Postgraduate Research & Practice Innovation Programs of Jiangsu Province, and Jiangsu Province Higher Education Teaching Reform Research Project (Grant No. 2025JGYB487).

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摘要： A highly sensitive, ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe (ROFP) is proposed and demonstrated. Our approach achieves a mass limit of detection of $\sim10$ aM based on the ROFP. A particular single-nucleotide mismatch sequence is also identified. The sensitivity of the proposed DNA biosensor is 1.51 nm/lgaM, about three-fold higher than using single-strand DNA probes (0.47 nm/lgaM) and with high specificity. The proposed ROFP has high compactness (with a length of $\sim3 $ mm) which is convenient for sample detection with small volume and complex gradients in small spaces with high sensitivity.

关键词: DNA biosensor, ultra-low detection limit, high sensitivity, reflective optical fiber probe, secondary interference, 3-D DNA nanostructure

Abstract: A highly sensitive, ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe (ROFP) is proposed and demonstrated. Our approach achieves a mass limit of detection of $\sim10$ aM based on the ROFP. A particular single-nucleotide mismatch sequence is also identified. The sensitivity of the proposed DNA biosensor is 1.51 nm/lgaM, about three-fold higher than using single-strand DNA probes (0.47 nm/lgaM) and with high specificity. The proposed ROFP has high compactness (with a length of $\sim3 $ mm) which is convenient for sample detection with small volume and complex gradients in small spaces with high sensitivity.

Key words: DNA biosensor, ultra-low detection limit, high sensitivity, reflective optical fiber probe, secondary interference, 3-D DNA nanostructure

中图分类号:  (Fiber optics)

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42.81.Pa (Sensors, gyros) 87.85.fk (Biosensors)