中国物理B ›› 2022, Vol. 31 ›› Issue (10): 107801-107801.doi: 10.1088/1674-1056/ac7215

• CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES • 上一篇    下一篇

A novel natural surface-enhanced fluorescence system based on reed leaf as substrate for crystal violet trace detection

Hui-Ju Cao(曹会菊)1, Hong-Wen Cao(曹红文)1, Yue Li(李月)1, Zhen Sun(孙祯)1, Yun-Fan Yang(杨云帆)1, Ti-Feng Jiao(焦体峰)2,†, and Ming-Li Wang(王明利)1,‡   

  1. 1. State Key Laboratory of Materials Science & Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University, Qinhuangdao 066004, China;
    2. Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China
  • 收稿日期:2022-04-25 修回日期:2022-05-16 出版日期:2022-10-16 发布日期:2022-09-27
  • 通讯作者: Ti-Feng Jiao, Ming-Li Wang E-mail:tfjiao@ysu.edu.cn;wml@ysu.edu.cn
  • 基金资助:
    Project supported by the National Natural Science Foundation of China (Grant Nos. 11674275, 21872119, 22072127, and 12104392) and the Science and Technology Project of Hebei Education Department, China (Grant No. ZD2019069 and QN2021142)).

A novel natural surface-enhanced fluorescence system based on reed leaf as substrate for crystal violet trace detection

Hui-Ju Cao(曹会菊)1, Hong-Wen Cao(曹红文)1, Yue Li(李月)1, Zhen Sun(孙祯)1, Yun-Fan Yang(杨云帆)1, Ti-Feng Jiao(焦体峰)2,†, and Ming-Li Wang(王明利)1,‡   

  1. 1. State Key Laboratory of Materials Science & Technology and Key Laboratory for Microstructural Material Physics of Hebei Province, School of Science, Yanshan University, Qinhuangdao 066004, China;
    2. Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China
  • Received:2022-04-25 Revised:2022-05-16 Online:2022-10-16 Published:2022-09-27
  • Contact: Ti-Feng Jiao, Ming-Li Wang E-mail:tfjiao@ysu.edu.cn;wml@ysu.edu.cn
  • Supported by:
    Project supported by the National Natural Science Foundation of China (Grant Nos. 11674275, 21872119, 22072127, and 12104392) and the Science and Technology Project of Hebei Education Department, China (Grant No. ZD2019069 and QN2021142)).

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摘要： The preparation of surface-enhanced fluorescence (SEF) substrates is often influenced by experimental strategies and factors such as the morphology and size of the nanostructures. In this study, using the natural reed leaves (RLs) without any special pretreatment as the substrate, metal silver is modified by magnetron sputtering technology to prepare a stable and efficient SEF system. The abundant “hedgehog-like”protrusions on the RL substrate surface can generate high-density “hot spots”, thus enhancement factor (EF) is enhanced up to 3345 times. The stability and reproducibility are verified in many measurements. The contribution of the intervention of silver nanostructure to the radiation attenuation process of fluorescent molecules is analyzed with the aid of Jablonski diagrams. Three-dimensional (3D) finite difference time domain (FDTD) simulates the spatial electric field and “hot spots”distribution of the substrate. The “hedgehog-like”protrusion structure generates multiple “hot spots”, which produce an excellent local surface plasmon resonance (LSPR) effect and provide higher fluorescence signal. Finally, RL/Ag-35 substrate is used to detect crystal violet (CV), and the detection limit is as low as 10-13 M. This “hedgehog-like”SEF substrate provides a new strategy for the trace detection of CV, which has a good practical application value.

关键词: surface-enhanced fluorescence, biological materials, reed leaf, hot spots

Abstract: The preparation of surface-enhanced fluorescence (SEF) substrates is often influenced by experimental strategies and factors such as the morphology and size of the nanostructures. In this study, using the natural reed leaves (RLs) without any special pretreatment as the substrate, metal silver is modified by magnetron sputtering technology to prepare a stable and efficient SEF system. The abundant “hedgehog-like”protrusions on the RL substrate surface can generate high-density “hot spots”, thus enhancement factor (EF) is enhanced up to 3345 times. The stability and reproducibility are verified in many measurements. The contribution of the intervention of silver nanostructure to the radiation attenuation process of fluorescent molecules is analyzed with the aid of Jablonski diagrams. Three-dimensional (3D) finite difference time domain (FDTD) simulates the spatial electric field and “hot spots”distribution of the substrate. The “hedgehog-like”protrusion structure generates multiple “hot spots”, which produce an excellent local surface plasmon resonance (LSPR) effect and provide higher fluorescence signal. Finally, RL/Ag-35 substrate is used to detect crystal violet (CV), and the detection limit is as low as 10-13 M. This “hedgehog-like”SEF substrate provides a new strategy for the trace detection of CV, which has a good practical application value.

Key words: surface-enhanced fluorescence, biological materials, reed leaf, hot spots

中图分类号:  (Optical properties of low-dimensional, mesoscopic, and nanoscale materials and structures)

  • 78.67.-n
78.55.-m (Photoluminescence, properties and materials) 73.20.-r (Electron states at surfaces and interfaces)