Cholesterol-induced deformation of the gramicidin A channel inhibiting potassium ion binding and transport

doi:10.1088/1674-1056/ad334e

中国物理B ›› 2024, Vol. 33 ›› Issue (5): 58701-058701.doi: 10.1088/1674-1056/ad334e

Cholesterol-induced deformation of the gramicidin A channel inhibiting potassium ion binding and transport

Pan Xiao(肖盼)^1,2, Yu Cao(曹宇)^1,2, Jin Zhu(朱瑾)^1,2, and Qing Liang(梁清)^1,2,†

1 Center for Statistical and Theoretical Condensed Matter Physics & Department of Physics, Zhejiang Normal University, Jinhua 321004, China;
2 Zhejiang Institute of Photoelectronics & Zhejiang Institute for Advanced Light Source, Zhejiang Normal University, Jinhua 321004, China

收稿日期:2024-02-04 修回日期:2024-02-26 接受日期:2024-03-13 出版日期:2024-05-20 发布日期:2024-05-20
通讯作者: Qing Liang E-mail:qliang@zjnu.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant No. 11674287) and the Zhejiang Provincial Natural Science Foundation of China (Grant No. LY19A040009).

Cholesterol-induced deformation of the gramicidin A channel inhibiting potassium ion binding and transport

Pan Xiao(肖盼)^1,2, Yu Cao(曹宇)^1,2, Jin Zhu(朱瑾)^1,2, and Qing Liang(梁清)^1,2,†

1 Center for Statistical and Theoretical Condensed Matter Physics & Department of Physics, Zhejiang Normal University, Jinhua 321004, China;
2 Zhejiang Institute of Photoelectronics & Zhejiang Institute for Advanced Light Source, Zhejiang Normal University, Jinhua 321004, China

Received:2024-02-04 Revised:2024-02-26 Accepted:2024-03-13 Online:2024-05-20 Published:2024-05-20
Contact: Qing Liang E-mail:qliang@zjnu.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant No. 11674287) and the Zhejiang Provincial Natural Science Foundation of China (Grant No. LY19A040009).

摘要/Abstract

摘要： Gramicidin A (gA) is a kind of antibiotic peptide produced by bacillus brevis and it can dimerize across lipid bilayers to form a monovalent cation channel. In this work, we investigate the impact of cholesterol in the lipid bilayer on the binding of potassium ions with the gA channel and the transport of the ions across the channel. The results indicate that cholesterol can significantly influence the conformational stability of the gA channel and cause the channel deformation which inhibits the potassium ion binding with the channel and transport across the channel. The work provides some molecular insights into understanding of influence of lipids on the activity of gA channel in both model membranes and plasma membranes of intact cells.

关键词: gramicidin A, lipid bilayer, molecular dynamics, interaction

Abstract: Gramicidin A (gA) is a kind of antibiotic peptide produced by bacillus brevis and it can dimerize across lipid bilayers to form a monovalent cation channel. In this work, we investigate the impact of cholesterol in the lipid bilayer on the binding of potassium ions with the gA channel and the transport of the ions across the channel. The results indicate that cholesterol can significantly influence the conformational stability of the gA channel and cause the channel deformation which inhibits the potassium ion binding with the channel and transport across the channel. The work provides some molecular insights into understanding of influence of lipids on the activity of gA channel in both model membranes and plasma membranes of intact cells.

Key words: gramicidin A, lipid bilayer, molecular dynamics, interaction

中图分类号: (Peptides)

87.14.ef

87.16.D- (Membranes, bilayers, and vesicles) 87.15.ap (Molecular dynamics simulation) 87.15.K- (Molecular interactions; membrane-protein interactions)

Pan Xiao(肖盼), Yu Cao(曹宇), Jin Zhu(朱瑾), and Qing Liang(梁清). Cholesterol-induced deformation of the gramicidin A channel inhibiting potassium ion binding and transport[J]. 中国物理B, 2024, 33(5): 58701-058701.

Pan Xiao(肖盼), Yu Cao(曹宇), Jin Zhu(朱瑾), and Qing Liang(梁清). Cholesterol-induced deformation of the gramicidin A channel inhibiting potassium ion binding and transport[J]. Chin. Phys. B, 2024, 33(5): 58701-058701.

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Cholesterol-induced deformation of the gramicidin A channel inhibiting potassium ion binding and transport

Cholesterol-induced deformation of the gramicidin A channel inhibiting potassium ion binding and transport

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