Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation

doi:10.1088/1674-1056/ab50fd

中国物理B ›› 2019, Vol. 28 ›› Issue (12): 128702-128702.doi: 10.1088/1674-1056/ab50fd

• INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY • 上一篇下一篇

Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation

Xin-Xin Zhang(张欣欣), Ming-Xia He(何明霞), Yu Chen(陈宇), Cheng Li(李程), Jin-Wu Zhao(赵晋武), Peng-Fei Wang(王鹏騛), Xin Peng(彭鑫)

1 The Center for Terahertz Waves, School of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China;
2 State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin 300072, China;
3 Department of Chemistry, School of Science, Tianjin University, Tianjin 300354, China;
4 School of Life Sciences, Tianjin University, Tianjin 300072, China

收稿日期:2019-08-13 修回日期:2019-10-11 出版日期:2019-12-05 发布日期:2019-12-05
通讯作者: Ming-Xia He E-mail:hhmmxx@tju.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant No. 61675151).

Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation

Xin-Xin Zhang(张欣欣)^1,2, Ming-Xia He(何明霞)^1,2, Yu Chen(陈宇)³, Cheng Li(李程)³, Jin-Wu Zhao(赵晋武)^1,2, Peng-Fei Wang(王鹏騛)^1,2, Xin Peng(彭鑫)⁴

1 The Center for Terahertz Waves, School of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China;
2 State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin 300072, China;
3 Department of Chemistry, School of Science, Tianjin University, Tianjin 300354, China;
4 School of Life Sciences, Tianjin University, Tianjin 300072, China

Received:2019-08-13 Revised:2019-10-11 Online:2019-12-05 Published:2019-12-05
Contact: Ming-Xia He E-mail:hhmmxx@tju.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant No. 61675151).

摘要/Abstract

摘要： Alkaline phosphatase (ALP) plays an integral role in the metabolism of liver and development of the skeleton in humans. To date, the interactions between different-duration terahertz (THz) radiation and ALP activities, as well as the influence mechanism are still unclear. In this study, using the para-nitro-phenyl-phosphate (pNPP) method, we detect changes in ALP activities during 40-minute THz radiation (0.1 THz, 13 mW/cm²). It is found that the activity of ALP decreases in the first 25 min, and subsequently increases in the later 15 min. Compared with the activity of ALP being heated, the results suggest that short-term terahertz radiation induces a decrease in enzyme activity through the non-thermal mechanism. In order to explore the non-thermal effects of THz radiation on ALP, we focus on the impacts of 0.1 THz radiation for 20 min on the activity of ALP in different concentrations. The results reveal that the activity of ALP decreases significantly after exposure to THz radiation. In addition, it could be deduced from fluorescence, ultraviolet-visible (UV-vis), and THz spectra results that THz radiation has induced changes in ALP structures. Our study unlocks non-thermal interactions between THz radiation and ALP, as well as suggests that THz spectroscopy is a promising technique to distinguish ALP structures.

关键词: THz radiation, alkaline phosphatase, non-thermal effects, spectroscopy, conformations

Abstract: Alkaline phosphatase (ALP) plays an integral role in the metabolism of liver and development of the skeleton in humans. To date, the interactions between different-duration terahertz (THz) radiation and ALP activities, as well as the influence mechanism are still unclear. In this study, using the para-nitro-phenyl-phosphate (pNPP) method, we detect changes in ALP activities during 40-minute THz radiation (0.1 THz, 13 mW/cm²). It is found that the activity of ALP decreases in the first 25 min, and subsequently increases in the later 15 min. Compared with the activity of ALP being heated, the results suggest that short-term terahertz radiation induces a decrease in enzyme activity through the non-thermal mechanism. In order to explore the non-thermal effects of THz radiation on ALP, we focus on the impacts of 0.1 THz radiation for 20 min on the activity of ALP in different concentrations. The results reveal that the activity of ALP decreases significantly after exposure to THz radiation. In addition, it could be deduced from fluorescence, ultraviolet-visible (UV-vis), and THz spectra results that THz radiation has induced changes in ALP structures. Our study unlocks non-thermal interactions between THz radiation and ALP, as well as suggests that THz spectroscopy is a promising technique to distinguish ALP structures.

Key words: THz radiation, alkaline phosphatase, non-thermal effects, spectroscopy, conformations

中图分类号: (Biophysical mechanisms of interaction)

87.50.uj

87.14.ej (Enzymes) 87.15.hp (Conformational changes)

张欣欣, 何明霞, 陈宇, 李程, 赵晋武, 王鹏騛, 彭鑫. Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation[J]. 中国物理B, 2019, 28(12): 128702-128702.

Xin-Xin Zhang(张欣欣), Ming-Xia He(何明霞), Yu Chen(陈宇), Cheng Li(李程), Jin-Wu Zhao(赵晋武), Peng-Fei Wang(王鹏騛), Xin Peng(彭鑫). Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation[J]. Chin. Phys. B, 2019, 28(12): 128702-128702.

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Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation

Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation

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