Cryo-ET bridges the gap between cell biology and structural biophysics

doi:10.1088/1674-1056/27/6/066803

中国物理B ›› 2018, Vol. 27 ›› Issue (6): 66803-066803.doi: 10.1088/1674-1056/27/6/066803

所属专题： TOPICAL REVIEW — Electron microscopy methods for emergent materials and life sciences

• TOPICAL REVIEW—Electron microscopy methods for the emergent materials and life sciences • 上一篇下一篇

Cryo-ET bridges the gap between cell biology and structural biophysics

Xiao-Fang Cheng(程小芳), Rui Wang(王睿), Qing-Tao Shen(沈庆涛)

1 iHuman Institute, ShanghaiTech University, Shanghai 201210, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;
3 School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

收稿日期:2018-01-02 修回日期:2018-04-23 出版日期:2018-06-05 发布日期:2018-06-05
通讯作者: Rui Wang, Qing-Tao Shen E-mail:wangrui1@shanghaitech.edu.cn;shenqt@shanghaitech.edu.cn
基金资助:
Project supported by the National Key Research and Development Program of China (Grant No.2017YFA0504800) and the Pujiang Talent Program (Grant No.17PJ1406700).

Cryo-ET bridges the gap between cell biology and structural biophysics

Xiao-Fang Cheng(程小芳)^1,2, Rui Wang(王睿)^1,2, Qing-Tao Shen(沈庆涛)^1,2,3

1 iHuman Institute, ShanghaiTech University, Shanghai 201210, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;
3 School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

Received:2018-01-02 Revised:2018-04-23 Online:2018-06-05 Published:2018-06-05
Contact: Rui Wang, Qing-Tao Shen E-mail:wangrui1@shanghaitech.edu.cn;shenqt@shanghaitech.edu.cn
Supported by:
Project supported by the National Key Research and Development Program of China (Grant No.2017YFA0504800) and the Pujiang Talent Program (Grant No.17PJ1406700).

摘要/Abstract

摘要：

Cryo-electron tomography (cryo-ET) is a cutting-edge technology providing three-dimensional in situ ultra-structural information of macromolecular machineries, organelles, and eukaryotic cells in their native environment at an unprecedented level of detail. Cryo-ET enables the direct observation of dynamic macromolecular architectures of bio-samples in their naturally occurring physiological state, without any harmful artifacts introduced by heavy metal staining, dehydration, and chemical fixation, which occur in traditional transmission electron microscopy. Over decades, cryo-ET has been providing insights into numerous aspects of cellular biology by revealing the pristinely preserved ultra-structures of different cellular components comprising the crowded and complex environment of the cell, thus, bridging the gap between cellular biology and structural biophysics. In this paper, we review the fundamentals of this technique, its recent advances in optics, detection devices, and computational algorithms. The enhancement of our understanding of structural cellular biology by combining these improvements, when integrated with other methods, such as cryo-focused ion beam milling, correlative light and electron microscopy, is discussed via a few examples from research groups worldwide. We also believe that cryo-ET applications in cell biology continue to provide fundamental insights into the field, revolutionizing structural biology itself.

关键词: sub-tomogram averaging, segmentation, direct detection, phase plate

Abstract:

Key words: sub-tomogram averaging, segmentation, direct detection, phase plate

中图分类号: (High-resolution transmission electron microscopy (HRTEM))

68.37.Og

68.37.Lp (Transmission electron microscopy (TEM)) 87.64.Ee (Electron microscopy)

程小芳, 王睿, 沈庆涛. Cryo-ET bridges the gap between cell biology and structural biophysics[J]. 中国物理B, 2018, 27(6): 66803-066803.

Xiao-Fang Cheng(程小芳), Rui Wang(王睿), Qing-Tao Shen(沈庆涛). Cryo-ET bridges the gap between cell biology and structural biophysics[J]. Chin. Phys. B, 2018, 27(6): 66803-066803.

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Cryo-ET bridges the gap between cell biology and structural biophysics

Cryo-ET bridges the gap between cell biology and structural biophysics

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