中国物理B ›› 2012, Vol. 21 ›› Issue (2): 20601-020601.doi: 10.1088/1674-1056/21/2/020601

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张洪艳1,杨立泉1,孟岚2,聂家财2,宁廷银1,刘卫敏1,孙嘉宇1,汪鹏飞1   

  • 收稿日期:2011-07-22 修回日期:2011-08-13 出版日期:2012-01-30 发布日期:2012-01-30
  • 通讯作者: 聂家财,jcnie@bnu.eud.cn;汪鹏飞,wangpf@mail.ipc.ac.cn E-mail:jcnie@bnu.eud.cn;wangpf@mail.ipc.ac.cn

Real time detection of antibody–antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system

Zhang Hong-Yan(张洪艳)a), Yang Li-Quan(杨立泉)a), Meng Lan(孟岚)b), Nie Jia-Cai(聂家财)b)†, Ning Ting-Yin(宁廷银)a), Liu Wei-Min(刘卫敏)a), Sun Jia-Yu(孙嘉宇)a), and Wang Peng-Fei(汪鹏飞)a)‡   

  1. a. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    b. Departments of Physics, Beijing Normal University, Beijing 100875, China
  • Received:2011-07-22 Revised:2011-08-13 Online:2012-01-30 Published:2012-01-30
  • Contact: Nie Jia-Cai,jcnie@bnu.eud.cn;Wang Peng-Fei,wangpf@mail.ipc.ac.cn E-mail:jcnie@bnu.eud.cn;wangpf@mail.ipc.ac.cn
  • Supported by:
    Project supported by the Instrument Developing Project of the Chinese Academy of Sciences (Grant No. YZ200740), the National Natural Science Foundation of China (Grant Nos. 60978034 and 10974019), and the National High Technology Research and Development Program of China (Grant No. 2009AA03Z318).

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Abstract: A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.

Key words: laser scanning confocal imaging-surface plasmon resonance, combination, complementation, real time

中图分类号:  (Other topics in metrology, measurements, and laboratory procedures)

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07.60.-j (Optical instruments and equipment) 42.30.-d (Imaging and optical processing) 87.15.km (Protein-protein interactions)