中国物理B ›› 2011, Vol. 20 ›› Issue (1): 10704-010704.doi: 10.1088/1674-1056/20/1/010704

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Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method

陆珩1, 戴俊1, 温娟1, 原昆1, 吕惠宾1, 金奎娟1, 周岳亮1, 杨国桢1, 王旭2   

  1. (1)Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China; (2)Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;Bioinformatics Laboratory and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
  • 收稿日期:2010-02-02 修回日期:2010-02-26 出版日期:2011-01-15 发布日期:2011-01-15
  • 基金资助:
    Project supported by the National Basic Research Program in China (Grant No. 2007CB935700).

Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method

Wang Xu(王旭)a)b), Lu Heng(陆珩)a), Dai Jun(戴俊)a), Wen Juan(温娟)a), Yuan Kun(原昆)a), Lü Hui-Bin(吕惠宾)a), Jin Kui-Juan(金奎娟)a), Zhou Yue-Liang(周岳亮) a), and Yang Guo-Zhen(杨国桢)a)†   

  1. a Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China; b Bioinformatics Laboratory and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2010-02-02 Revised:2010-02-26 Online:2011-01-15 Published:2011-01-15
  • Supported by:
    Project supported by the National Basic Research Program in China (Grant No. 2007CB935700).

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摘要： We successfully conduct the label-free and real-time detection of the interactions between epoxy groups and rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide, and between the hybridization of 5' CTT CAG GTC ATG AGC CTG AT 3' and its complementary 3' GAA GTC CAG TAC TCG GAC TA 5' oligonucleotide, by the oblique-incidence reflectivity difference (OI-RD) method. The dynamic curves of OI-RD signals, corresponding to the kinetic processes of biomolecular combination or hybridization, are acquired. In our case, the combination of epoxy groups with rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide need almost one and a half hours and about two hundred seconds, respectively; and the hybridization of the two oligonucleotides needs about five hundred seconds. The experimental results show that the OI-RD is a promising method for the real-time and label-free detection of biomolecular interactions.

关键词: label-free detection, OI-RD, biomolecular reactions

Abstract: We successfully conduct the label-free and real-time detection of the interactions between epoxy groups and rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide, and between the hybridization of 5' CTT CAG GTC ATG AGC CTG AT 3' and its complementary 3' GAA GTC CAG TAC TCG GAC TA 5' oligonucleotide, by the oblique-incidence reflectivity difference (OI-RD) method. The dynamic curves of OI-RD signals, corresponding to the kinetic processes of biomolecular combination or hybridization, are acquired. In our case, the combination of epoxy groups with rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide need almost one and a half hours and about two hundred seconds, respectively; and the hybridization of the two oligonucleotides needs about five hundred seconds. The experimental results show that the OI-RD is a promising method for the real-time and label-free detection of biomolecular interactions.

Key words: label-free detection, OI-RD, biomolecular reactions

中图分类号:  (Polarimeters and ellipsometers)

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