Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice

doi:10.1088/1009-1963/12/10/319

中国物理B ›› 2003, Vol. 12 ›› Issue (10): 1159-1165.doi: 10.1088/1009-1963/12/10/319

Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice

任兆玉¹, 侯洵², 辛越勇³, 许晓明⁴, 王水才⁵, 贺俊芳⁵

(1)Institute of Photonics & Photo-Technology, Northwest University, Xi'an 710068, China; (2)Institute of Photonics & Photo-Technology, Northwest University, Xi'an 710068, China; State Key Laboratory of Transience Optics Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710068, China; (3)Laboratory of Photosynthesis Basic Research, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; (4)Laboratory of Photosynthesis Research, Nanjing Agricultural University, Nanjing 210095, China; (5)State Key Laboratory of Transience Optics Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710068, China

收稿日期:2003-01-09 修回日期:2003-05-23 出版日期:2003-10-16 发布日期:2005-03-16
基金资助:
Project supported by the State Key Development Program for Basic Research of China (Grant No G1998010100).

Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice

Ren Zhao-Yu (任兆玉)^a, Xu Xiao-Ming (许晓明)^b, Wang Shui-Cai (王水才)^c, Xin Yue-Yong (辛越勇)^d, He Jun-Fang (贺俊芳)^c, Hou Xun (侯洵)^ac

^a Institute of Photonics & Photo-Technology, Northwest University, Xi'an 710068, China; ^b Laboratory of Photosynthesis Research, Nanjing Agricultural University, Nanjing 210095, China; ^c State Key Laboratory of Transience Optics Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710068, China; ^d Laboratory of Photosynthesis Basic Research, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2003-01-09 Revised:2003-05-23 Online:2003-10-16 Published:2005-03-16
Supported by:
Project supported by the State Key Development Program for Basic Research of China (Grant No G1998010100).

摘要/Abstract

摘要： A high yielding rice variety mutant (Oryza sativa L., Zhenhui 249) with low chlorophyll b (Chl b) has been discovered in natural fields. It has a quality character controlled by a pair of recessive genes (nuclear gene). The partial loss of Chl b in content affects the efficiency of light harvest in a light harvest complex (LHC), thus producing the difference of the exciting energy transfer and the efficiency of photochemistry conversion between the mutant and wild-type rice in photosynthetic unit. The efficiency of utilizing light energy is higher in the mutant than that in the wild-type rice relatively. For further discussion of the above-mentioned difference and learning about the mechanism of the increase in the photochemical efficiency of the mutant, the pico-second resolution fluorescence spectrum measurement with delay-frame-scanning single photon counting technique is adopted. Thylakoid membranes of the mutant and the wild-type rice are excited by an Ar^+ laser with a pulse width of 120 ps, repetition rate of 4 MHz and wavelength of 514 nm. Compared with the time and spectrum property of exciting fluorescence, conclusions of those ultrafast dynamic experiments are: 1) The speeds of the exciting energy transferred in photo-system I are faster than that in photo-system II in both samples. 2) The speeds of the exciting energy transfer of mutant sample are faster than those of the wild-type. This might be one of the major reasons why the efficiency of photosynthesis is higher in mutant than that in the wild-type rice.

Abstract: A high yielding rice variety mutant (Oryza sativa L., Zhenhui 249) with low chlorophyll b (Chl b) has been discovered in natural fields. It has a quality character controlled by a pair of recessive genes (nuclear gene). The partial loss of Chl b in content affects the efficiency of light harvest in a light harvest complex (LHC), thus producing the difference of the exciting energy transfer and the efficiency of photochemistry conversion between the mutant and wild-type rice in photosynthetic unit. The efficiency of utilizing light energy is higher in the mutant than that in the wild-type rice relatively. For further discussion of the above-mentioned difference and learning about the mechanism of the increase in the photochemical efficiency of the mutant, the pico-second resolution fluorescence spectrum measurement with delay-frame-scanning single photon counting technique is adopted. Thylakoid membranes of the mutant and the wild-type rice are excited by an Ar^+ laser with a pulse width of 120 ps, repetition rate of 4 MHz and wavelength of 514 nm. Compared with the time and spectrum property of exciting fluorescence, conclusions of those ultrafast dynamic experiments are: 1) The speeds of the exciting energy transferred in photo-system I are faster than that in photo-system II in both samples. 2) The speeds of the exciting energy transfer of mutant sample are faster than those of the wild-type. This might be one of the major reasons why the efficiency of photosynthesis is higher in mutant than that in the wild-type rice.

Key words: rice, photosynthesis, ultrafast process, time constant, exciting energy transfer

中图分类号: (Photochemistry)

82.50.-m

84.60.-h (Direct energy conversion and storage)

任兆玉, 许晓明, 王水才, 辛越勇, 贺俊芳, 侯洵. Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice[J]. 中国物理B, 2003, 12(10): 1159-1165.

Ren Zhao-Yu (任兆玉), Xu Xiao-Ming (许晓明), Wang Shui-Cai (王水才), Xin Yue-Yong (辛越勇), He Jun-Fang (贺俊芳), Hou Xun (侯洵). Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice[J]. Chinese Physics, 2003, 12(10): 1159-1165.

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Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice

Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice

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