Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mimicking microenvironment of tumor<xref rid="cpb_27_12_128703_fn1" ref-type="fn">*</xref><fn id="cpb_27_12_128703_fn1"><label>*</label><p>Project supported by the National Natural Science Foundation of China (Grant Nos. 11474345, 11674043, and 11604030) and the Fundamental and Advanced Research Program of Chongqing (Grant No. cstc2018jcyjAX0338).</p></fn>

Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mimicking microenvironment of tumor**

Project supported by the National Natural Science Foundation of China (Grant Nos. 11474345, 11674043, and 11604030) and the Fundamental and Advanced Research Program of Chongqing (Grant No. cstc2018jcyjAX0338).

Li Yuenan¹, Hai Miaomiao¹, Zhao Yu², Lv Yalei¹, He Yi¹, Chen Guo¹, Liu Liyu¹, Liu Ruchuan^{1, ‡}, Zhang Guigen^{2, §}

(color online) Cell viability and proliferation. Different packing density of cells encapsulated in the core of 3D hydrogel microspheres at different ratio of V_A2 to V_A1 of (a) 1:3 and (b) 1:2, respectively. The net flow rate of aqueous phase (V_A1+V_A2) was maintained at 120 μL/h. The scale bar is 40 μm. (c) Statistical results of viability of MDA-MB-231 cells encapsulated in alginate + Matrigel + Collagen, alginate + Matrigel, alginate + Collagen, and alginate only hydrogel for 8 days. 3D hydrogel environment with the addition of extracellular matrix components including both Collagen I and reconstituted basement membrane provides a better growth micro-environment for cells compared with those containing only one kind of hydrogel material.