Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mimicking microenvironment of tumor<xref rid="cpb_27_12_128703_fn1" ref-type="fn">*</xref><fn id="cpb_27_12_128703_fn1"><label>*</label><p>Project supported by the National Natural Science Foundation of China (Grant Nos. 11474345, 11674043, and 11604030) and the Fundamental and Advanced Research Program of Chongqing (Grant No. cstc2018jcyjAX0338).</p></fn>

Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mimicking microenvironment of tumor**

Project supported by the National Natural Science Foundation of China (Grant Nos. 11474345, 11674043, and 11604030) and the Fundamental and Advanced Research Program of Chongqing (Grant No. cstc2018jcyjAX0338).

Li Yuenan¹, Hai Miaomiao¹, Zhao Yu², Lv Yalei¹, He Yi¹, Chen Guo¹, Liu Liyu¹, Liu Ruchuan^{1, ‡}, Zhang Guigen^{2, §}

(color online) Droplets with tunable sizes are generated using the chip by experiment and simulation. (a) With increasing flow rates of oil phase 2, droplet size decreases. The scale bar is 200 μm. (b) Simulation results of dynamics of droplet generation. The flow rate of the oil phase 2 (V_O2) and aqueous phase (V_A) were 300 μL/h and 120 μL/h, respectively. (c) Simulation and experimental results for the influence of the flow rate of oil phase 2 on the droplet size. Both simulation results and experimental measurement show similar effect: With increased flow rates of oil 2, droplet size decreased.