The determinant factors for map resolutions obtained using CryoEM single particle imaging method<xref rid="cpb_27_12_128702fn1" ref-type="fn">*</xref><fn id="cpb_27_12_128702fn1"><label>*</label><p>Project supported by the National Natural Science Foundation of China (Grant Nos. 11774011, 11434001, U1530401, and U1430237).</p></fn>

The determinant factors for map resolutions obtained using CryoEM single particle imaging method**

Project supported by the National Natural Science Foundation of China (Grant Nos. 11774011, 11434001, U1530401, and U1430237).

Wang Yihua¹, Yu Daqi¹, Ouyang Qi¹, Liu Haiguang^{2, †}

(color online) The influence of thermal fluctuation to the structure resolution. (a) The relationship between the resolution and number of projections at various thermal fluctuation levels. The thermal fluctuation is quantified using the average square root-mean-square-derivation 〈RMSD² 〉 of the structure ensemble. All the projections were simulated with Gaussian noise with an SNR of 1.00. The R-square values of the fittings to the GN model are approximately 0.96. (b) The five datasets were fitted using a single set of parameters with an R-square of 0.98 using Eq. (5).