The determinant factors for map resolutions obtained using CryoEM single particle imaging method<xref rid="cpb_27_12_128702fn1" ref-type="fn">*</xref><fn id="cpb_27_12_128702fn1"><label>*</label><p>Project supported by the National Natural Science Foundation of China (Grant Nos. 11774011, 11434001, U1530401, and U1430237).</p></fn>

The determinant factors for map resolutions obtained using CryoEM single particle imaging method**

Project supported by the National Natural Science Foundation of China (Grant Nos. 11774011, 11434001, U1530401, and U1430237).

Wang Yihua¹, Yu Daqi¹, Ouyang Qi¹, Liu Haiguang^{2, †}

(color online) The two models used for CryoEM single particle imaging data simulations. (a) Gaussian noise model: noises were added to the simulation data to study the influence of noise on model resolution. (b) Thermal fluctuation model: a structure ensemble was first generated to mimic the structure heterogeneity. Each single particle projection was simulated based on a randomly picked structure from the ensemble. Three representative models for GroEL are shown in panel (b): the original structure is in gray; the yellow and blue structures were generated using normal mode perturbations.