Probing conformational change of T7 RNA polymerase and DNA complex by solid-state nanopores |
(color online) Current traces captured by Axon 200B for electrolyte (a), DNA promoter (b), T7 RNAP (c), and complex (d) obtained by a 10 nm nanopore in a condition of 0.15 M NaCl, pH = 8, and applied voltage of 100 mV. (e) Scatter plot of ΔG vs log dwell-times (td) for T7 RNAP with a 10 nm nanopore and applied voltage 100 mV. |