Cryo-ET bridges the gap between cell biology and structural biophysics<xref rid="cpb_27_6_066803fn1" ref-type="fn">*</xref> <fn id="cpb_27_6_066803fn1"> <label>*</label> <p>Project supported by the National Key Research and Development Program of China (Grant No. 2017YFA0504800) and the Pujiang Talent Program (Grant No. 17PJ1406700).</p> </fn>

Cryo-ET bridges the gap between cell biology and structural biophysics* *

Project supported by the National Key Research and Development Program of China (Grant No. 2017YFA0504800) and the Pujiang Talent Program (Grant No. 17PJ1406700).

Cheng Xiao-Fang^{1, 2}, Wang Rui^{1, 2, †}, Shen Qing-Tao^{1, 2, 3, ‡}

Future of cryo-ET. Cryo-ET alone has a variety of limitations. By integration with other approaches, such as CLEM, cryo-FIB milling, it can target any interior structures within any types of cell. The advancements in optics, such as the VPP, spherical aberration corrector and energy filter, the improvements in detection devices, such as direct detection, K2 summit electron counting, and also better computational algorithms, such as SIRT, ICON, 3D-CTF correction, and automatic segmentation, enable high-resolution imaging of any macromolecular complexes, cellular events within any type of cell, in the future. Modified with permission from Wagner et al., 2017.