Cryo-ET bridges the gap between cell biology and structural biophysics<xref rid="cpb_27_6_066803fn1" ref-type="fn">*</xref> <fn id="cpb_27_6_066803fn1"> <label>*</label> <p>Project supported by the National Key Research and Development Program of China (Grant No. 2017YFA0504800) and the Pujiang Talent Program (Grant No. 17PJ1406700).</p> </fn>

Cryo-ET bridges the gap between cell biology and structural biophysics* *

Project supported by the National Key Research and Development Program of China (Grant No. 2017YFA0504800) and the Pujiang Talent Program (Grant No. 17PJ1406700).

Cheng Xiao-Fang^{1, 2}, Wang Rui^{1, 2, †}, Shen Qing-Tao^{1, 2, 3, ‡}

(color online) TEM Study of nuclear pore complex (NPC). (a) Callan and Tomlin isolated the nuclear membrane of the oocyte of Xenopus laevis, and reported orderly array pores in the outer layer by TEM for the first time in 1950. (b) Using the ultra-thin sectioning technique, Bahr and Beermann reported on the nuclear membrane of salivary glands. (c) Faberge isolated the nuclei of Oocytes of the new Taricha granulosa, and directly demonstrated the eight-fold symmetry of the nuclear pores. (d) Akey and Radermacher isolated the Oocytes of Xenopus, and solved the 3D structure of NPC by Cryo-EM in 1993, with the resolution of 25 Å. (e) Composite structure of the human NPC acquired by docking all available single or subcomplex crystal structures into the cryo-ET map of the intact human NPC (EMD-3103).