Cryo-ET bridges the gap between cell biology and structural biophysics<xref rid="cpb_27_6_066803fn1" ref-type="fn">*</xref> <fn id="cpb_27_6_066803fn1"> <label>*</label> <p>Project supported by the National Key Research and Development Program of China (Grant No. 2017YFA0504800) and the Pujiang Talent Program (Grant No. 17PJ1406700).</p> </fn>

Cryo-ET bridges the gap between cell biology and structural biophysics* *

Project supported by the National Key Research and Development Program of China (Grant No. 2017YFA0504800) and the Pujiang Talent Program (Grant No. 17PJ1406700).

Cheng Xiao-Fang^{1, 2}, Wang Rui^{1, 2, †}, Shen Qing-Tao^{1, 2, 3, ‡}

(color online) Overview of sub-tomogram averaging. Sub-tomograms are extracted from the tomogram. Each sub-tomogram contains a “repeating particle” of interest. Sub-tomograms are rotationally and translationally aligned against a reference. Then, the aligned sub-tomograms are averaged to generate a new reference. Then, the new reference is used for alignment of the sub-tomograms again. This procedure is repeated until the reference stabilizes. Modified with permission from Briggs et al., 2013.