(color online) Spatially-resolved EELS analysis of Σ5 (310)/[001] GB in SrTiO₃ bicrystal: (a) high-resolution TEM image; (b) schematics of the experimental setups for two analytical modes, spectrum-profiling, and ELNES-separation; (c) spectrum-profiling presented in 2D (above) and 3D (below) view; (d) spectral processing by ELNES-separation to retract exclusive spectrum of GB layer (red) from the two spectra A (purple) and B (blue), measured in scanning frames on and off this GB (more details in the text).