(color online) The first near-atomic resolution structure (ribosome) determined by single particle cryo-EM by software RELION.^[11] (a) is an original photo; (b) is the classification of different complexes/conformers existing in the original photos; (c) displays the resolution assessment by FSC 0.143 for two ribosome complexes (P/E tRNA and P/I tRNA complexes) with resolutions 4.3 Å and 6.6 Å, respectively; (d) display structural model (colored ribbons/sticks) fitting into EM density maps (grey mesh). Figure from Ref. [11] by courtesy of Sjors Scheres (MRC-LMB, Cambridge, UK)