Structural biology revolution led by technical breakthroughs in cryo-electron microscopy*

Project supported by the National Key Research and Development Program of China (Grant No. 2017YFA0504700) and the National Natural Science Foundation of China (Grant Nos. 31570732 and 31770785).

Yin Chang-Cheng
       

(color online) The first near-atomic resolution structure (TRPV1) determined by single particle cryo-EM recorded by a DDD.[5] (a) An original photo; (b) the power spectrum (Fourier transform) of image after correction for image shift; (c) three typical projection views (2D averaged images) of TRPV1 with detailed structural information; (d)–(f) display the reconstructed density maps of TRPV1 in side (d and e), bottom (g), and top (f) views. The protein structural features (α-helices and β-sheets) are clearly identifiable. Figure from Ref. [5] by courtesy of Yifan Cheng (UCSF, USA).