Computing methods for icosahedral and symmetry-mismatch reconstruction of viruses by cryo-electron microscopy
Zhu Bin1, Cheng Lingpeng2, †, Liu Hongrong1, ‡
       

(color online) The process of genome image extraction. (a) The raw image is well aligned after a high resolution map of the capsid is reconstructed. (b) The annulus information of capsid shell can be determined from the raw image. (c) The particleʼs projection can be projected from high resolution map with CTF correction. (d) Subsequently, the annulus information of capsid shell can be determined from projection after masking of the inner information. (e) The information on the annulus of capsid shell is used to fit the same signal on the raw image and the projection, and the genome image is extracted by subtracting the capsid from the raw image. (f) The capsid information disappears on the genome image.