(color online) Flow chart of a symmetry-mismatch reconstruction. The procedure comprises three steps: nonicosahedral information extraction (black lines), initial asymmetry model generation (red lines), and symmetry-mismatch orientation determination and reconstruction (blue lines). The input data (grey lines) include raw particle images whose centers are shifted to image centers (0, 0), their icosahedral orientations for each particle image, and the 3D density map of the icosahedral capsid.