Influence of fluorescence time characteristics on the spatial resolution of CW-stimulated emission depletion microscopy

Qin Haiyun^{1, †}, Zhao Wei^{1, †}, Zhang Chen¹, Liu Yong², Wang Guiren³, Wang Kaige^{1, ‡}

(color online) Influence of the time constants on the FWHM of I eff ( x ) , i.e., Δ x n . The computation is based on case 2. (a) Influence of the rate of fluorescence, where k Q = 10 9 s − 1 and k vib = 10 12 s − 1 . (b) Influence of the rate of quenching, where k fl = 3 × 10 8 s − 1 and k vib = 10 12 s − 1 . (c) Influence of the rate of relaxation, where k fl = 3 × 10 8 s − 1 and k Q = 10 9 s − 1 .