Influence of fluorescence time characteristics on the spatial resolution of CW-stimulated emission depletion microscopy

Qin Haiyun^{1, †}, Zhao Wei^{1, †}, Zhang Chen¹, Liu Yong², Wang Guiren³, Wang Kaige^{1, ‡}

(color online) (a) Comparison of the experimental and numerical results summarized in Table 1. (b) Comparison of the numerical results with the experimental results from Luo et al.,^[17] where 405 nm ( 50 μ W ) and 532 nm lasers were used as excitation and STED beams, respectively. The fluorescent dye used is Atto 390. I f 0 and I f represent the fluorescent signals before and after depletion, respectively. Here, k fl = 2 × 10 8 s − 1 (official data from ATTO-TEC), k Q = 4 × 10 9 s − 1 (nonlinearly fitted by the experimental data), and k vib = 10 12 s − 1 .