Influence of fluorescence time characteristics on the spatial resolution of CW-stimulated emission depletion microscopy
Qin Haiyun1, †, Zhao Wei1, †, Zhang Chen1, Liu Yong2, Wang Guiren3, Wang Kaige1, ‡
       

(color online) (a) Coordinate system of the simulation. rp, φp, and zp are the radial, azimuthal, and axial components in the cylindrical coordinates, respectively. θ denotes the angle between the incident light and the optical axis. AL represents an achromatic lens. z is the axial direction of the incident beam. x and y are the axes perpendicular to the optical axis. (b) Illustration of the excitation beam (green), STED beam (red), and the resulting I eff x (yellow). Here, experimental case 2 is plotted for reference.