(color online) Results of the probability optimization algorithm for constant loading rate simulations. Optimized parameters (a) , (b) , (c) , and (d) are shown as functions of the average transition number from simulations of different time lengths. The error bar shows the standard deviation of results from 100 simulations of the same time length. The red lines are set values of simulations: pN, s⁻¹, nm, nm.