Bloom’s syndrome protein unfolding G-quadruplexes in two pathways

Zhao Zhen-Ye^{1, 2}, Xu Chun-Hua^{1, 2}, Shi Jing³, Li Jing-Hua⁴, Ma Jian-Bing^{1, 2}, Jia Qi^{1, 2}, Ma Dong-Fei^{1, 2}, Li Ming^{1, 2}, Lu Ying^{1, 2, †}

(color online) G4 on the loading overhang hindering BLM unwinding DNA duplex. (a) and 2(b) A typical FRET trace of BLM unwinding a DNA duplex without (a) and with (b) a G4 on the 3′ overhang. The schematic diagrams of the two substrates are shown in the inset of panel (c). (c) Comparison between the fractions of duplexes with and without a G4 unwound by BLM. Error = s.e.m.