Direct observation of <italic>λ</italic>-DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Direct observation of λ-DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Yang Fengyun¹, Wang Kaige^{1, †,}, Sun Dan¹, Zhao Wei^{1, 2}, Wang Hai-qing¹, He Xin¹, Wang Gui-ren², Bai Jin-tao¹

Around the threshold electric field, E = 5.3×10³ V/m (5 μm) and E = 4.0×10³ V/m (10 μm), the velocity profile of DNA molecules reversal transferring in the channel with different inner diameter (a) 5 μm and (b) 10 μm.