Direct observation of <italic>λ</italic>-DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Direct observation of λ-DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Yang Fengyun¹, Wang Kaige^{1, †,}, Sun Dan¹, Zhao Wei^{1, 2}, Wang Hai-qing¹, He Xin¹, Wang Gui-ren², Bai Jin-tao¹

The schematic diagram of deformation of DNA molecules translocating from the entrance into the inside of the microchannel. (a) When both ends of the DNA molecules are in the same side that is along the direction of fluid flow, they will be stretched by the viscosity of the fluid, (b) when the initial state of both ends of the DNA molecule is perpendicular to the direction of the flow, the conformation of DNA molecules enters into the channel.