Comparison of ligand migration and binding in heme proteins of the globin family
Nienhaus Karina) , Ulrich Nienhaus G.†a), b), c) 
Comparison of ligand migration and binding in heme proteins of the globin family |
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Nienhaus Karin
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| (a) Active site structures of Ngb in the bishistidine- hexacoordinate (red, pdb code 1Q1F) and CO-ligated (black, pdb code 1W92) states. (b) CO rebinding in Ngb monitored at 436 nm and 293 K. Closed symbols: 1-bar CO; open symbols: 0.05-bar CO. Inset: geminate phase recorded at 275 K. (c) Contour plot showing the time dependence (30 ns–3 s) of the photolysis difference spectrum of NgbCO (400 nm–450 nm). Schematic depictions of photolysis difference spectra at 10 μs (blue line) and 100 ms (red line) after the flash are included. Dotted black line: kinetics measured at 436 nm. |
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