Comparison of ligand migration and binding in heme proteins of the globin family |
(a) Essential features of the HbICO dimer. (b) Flash photolysis kinetics of CO rebinding (at 0.05-bar CO) in HbICO variants dissolved in buffer (closed symbols) and 75%/25% glycerol/buffer (open symbols), monitored at 436 nm and 20 °C. Black, wild-type HbI; green, I25W HbI; red: I114F HbI; purple, H69L HbI. |
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