Catch-bond behavior of DNA condensate under tension |
(a) Unfolding time traces obtained from experimental folding and unfolding cycles of a DNA molecule in the presence of 0.2-mM cobalt hexamine wherein the condensates could be completely unfolded at 10 pN–30 pN. (b) In the presence of 2-mM cobalt hexamine, a folded DNA molecule was stretched at different forces. At 15 pN–18 pN, complete unfolding was observed. At 22 pN–33 pN, the DNA was locked in a shorter conformation. Complete unfolding occurred at > 33 pN. |