Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition
Liu Li, Wu Lie, Zeng Li, Jiang Xiu-E†
       
Surface-enhanced infrared adsorption spectroscopy (SEIRAS) monitoring of the formation of cardiolipin-phosphatidylcholine (CL_PC) lipid membranes on a series of 1-dodecanethiol (DT)-gold surfaces via vesicle fusion of 1 mg/mL CL_PC (1:4) vesicles onto DT(10) (curve a ), DT(20) (curve b ), and DT(40) (curve c ) monolayers. The inset shows the second-derivative spectra. All spectra are at their individual time point of saturation adsorption.