Impact of microsecond-pulsed plasma-activated water on papaya seed germination and seedling growth

doi:10.1088/1674-1056/ac904e

中国物理B ›› 2022, Vol. 31 ›› Issue (12): 128201-128201.doi: 10.1088/1674-1056/ac904e

Impact of microsecond-pulsed plasma-activated water on papaya seed germination and seedling growth

Deng-Ke Xi(席登科)¹, Xian-Hui Zhang(张先徽)², Si-Ze Yang(杨思泽)², Seong Shan Yap(叶尚姗)³, Kenji Ishikawa(石川健治)⁴, Masura Hori (堀勝)⁴, and Seong Ling Yap(叶尚凌)^1,4,†

1 Plasma Technology Research Center, Department of Physics, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;
2 Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Fujian Engineering Research Center for EDA, Fujian Provincial Key Laboratory of Electromagnetic Wave Science and Detection Technology, Xiamen Key Laboratory of Multiphysics Electronic Information, Institute of Electromagnetics and Acoustics, Xiamen University, Xiamen 361005, China;
3 Department of Physics, Xiamen University Malaysia, Selangor Darul Ehsan 43900, Malaysia;
4 Center for Low-temperature Plasma Sciences, Nagoya University, Nagoya 464-8603 Aichi, Japan

收稿日期:2022-07-29 修回日期:2022-08-30 接受日期:2022-09-08 出版日期:2022-11-11 发布日期:2022-11-28
通讯作者: Seong Ling Yap E-mail:yapsl@um.edu.my
基金资助:
The authors from University of Malaya acknowledge the support from the Ministry of Higher Education Malaysia for the Fundamental Research Project (Grant Nos. FRGS/1/2018/STG02/UM/02/8 and IIRG006A-19FNW). Project supported by the National Natural Science Foundation of China (Grant No. 51877184).

Impact of microsecond-pulsed plasma-activated water on papaya seed germination and seedling growth

1 Plasma Technology Research Center, Department of Physics, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;
2 Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Fujian Engineering Research Center for EDA, Fujian Provincial Key Laboratory of Electromagnetic Wave Science and Detection Technology, Xiamen Key Laboratory of Multiphysics Electronic Information, Institute of Electromagnetics and Acoustics, Xiamen University, Xiamen 361005, China;
3 Department of Physics, Xiamen University Malaysia, Selangor Darul Ehsan 43900, Malaysia;
4 Center for Low-temperature Plasma Sciences, Nagoya University, Nagoya 464-8603 Aichi, Japan

Received:2022-07-29 Revised:2022-08-30 Accepted:2022-09-08 Online:2022-11-11 Published:2022-11-28
Contact: Seong Ling Yap E-mail:yapsl@um.edu.my
Supported by:
The authors from University of Malaya acknowledge the support from the Ministry of Higher Education Malaysia for the Fundamental Research Project (Grant Nos. FRGS/1/2018/STG02/UM/02/8 and IIRG006A-19FNW). Project supported by the National Natural Science Foundation of China (Grant No. 51877184).

摘要/Abstract

摘要： The seed of Carica papaya consists of a hard shell-like testa with inhibitors in vivo causing slow, erratic and asynchronous germination. In this work, plasma-activated water prepared by microsecond-pulsed plasma jets (μPAW) was applied to treat papaya seeds. The μPAW after plasma activation of 30 min was about 40 ℃. The reactive species such as NO₂, NO₃, and H₂O₂ in the μPAW activated from deionized water were measured and correlated to the seed germination rate and the seedling growth performance. The μPAW-treated papaya seed achieved a higher germination rate of 90%, which is 26% higher than the control group using deionized water. Comparing the results with a hot water (40 ℃) reference group showed that the reactive species in μPAW played primary roles in germination improvement, with little effect caused by the heat shock. The μPAW also sterilized the treated seeds, reducing the germination stress. The morphological change in the seeds was observed by SEM, showing an effect of physical etching after treatment promoting seed imbibition. The biochemical mechanism of the seed germination was deduced with reference to the evolution of surface chemistry, functional groups, and ABA content. The accelerated seed metabolism observed was corresponded to the chemical modification pathway. Besides, early seedlings developed from treated seeds were observed to be healthy, grow more leaves, and have better root structures. The content of MDA in the treated papaya seedlings decreased along with increased SOD and higher ion concentration. The μPAW that can be prepared at atmospheric pressure for bulk production offers a low-risk and cost-effective seed priming technology that may significantly increase the production of agricultural crops.

关键词: plasma-activated water, non-thermal plasma, microbial inactivation, seed metabolism

Abstract: The seed of Carica papaya consists of a hard shell-like testa with inhibitors in vivo causing slow, erratic and asynchronous germination. In this work, plasma-activated water prepared by microsecond-pulsed plasma jets (μPAW) was applied to treat papaya seeds. The μPAW after plasma activation of 30 min was about 40 ℃. The reactive species such as NO₂, NO₃, and H₂O₂ in the μPAW activated from deionized water were measured and correlated to the seed germination rate and the seedling growth performance. The μPAW-treated papaya seed achieved a higher germination rate of 90%, which is 26% higher than the control group using deionized water. Comparing the results with a hot water (40 ℃) reference group showed that the reactive species in μPAW played primary roles in germination improvement, with little effect caused by the heat shock. The μPAW also sterilized the treated seeds, reducing the germination stress. The morphological change in the seeds was observed by SEM, showing an effect of physical etching after treatment promoting seed imbibition. The biochemical mechanism of the seed germination was deduced with reference to the evolution of surface chemistry, functional groups, and ABA content. The accelerated seed metabolism observed was corresponded to the chemical modification pathway. Besides, early seedlings developed from treated seeds were observed to be healthy, grow more leaves, and have better root structures. The content of MDA in the treated papaya seedlings decreased along with increased SOD and higher ion concentration. The μPAW that can be prepared at atmospheric pressure for bulk production offers a low-risk and cost-effective seed priming technology that may significantly increase the production of agricultural crops.

Key words: plasma-activated water, non-thermal plasma, microbial inactivation, seed metabolism

中图分类号: (Chemical kinetics and dynamics)

82.20.-w

52.75.-d (Plasma devices) 92.20.jb (Bacteria, microbiology and microbial ecology) 87.15.-v (Biomolecules: structure and physical properties)

Deng-Ke Xi(席登科), Xian-Hui Zhang(张先徽), Si-Ze Yang(杨思泽), Seong Shan Yap(叶尚姗), Kenji Ishikawa(石川健治), Masura Hori (堀勝), and Seong Ling Yap(叶尚凌). Impact of microsecond-pulsed plasma-activated water on papaya seed germination and seedling growth[J]. 中国物理B, 2022, 31(12): 128201-128201.

参考文献

[1] 2021 FAO
[2] Guorong D, Mingjun L, Fengwang M and Dong L 2009 Food Chem. 113 557
[3] Welde Y and Worku A 2018 J. Med. Plants Res. 6 127
[4] Rahman A 2013 Adv. Nat. Sci. 6 026
[5] Koornneef M, Bentsink L and Hilhorst H 2002 Curr. Opin. Plant Biol. 5 33
[6] Wood C B, Pritchard H W and Amritphale D 2000 Seed Sci. Res. 10 135
[7] Webster R E, Waterworth W M, Stuppy W, West C E, Ennos R, Bray C M and Pritchard H W 2016 J. Exp. Bot. 67 6373
[8] Debeaujon I, Léon-Kloosterziel K M and Koornneef M 2000 Plant Physiol. 122 403
[9] Reyes M N, Perez A and Cuevas J 1980 J. Agric. Univ. Puerto Rico 64 164
[10] Chow Y J and Lin C H 1991 Seed Sci. Technol. 19 167
[11] Garciarrubio A, Legaria J P and Covarrubias A A 1997 Planta 203 182
[12] Salomão A N and Mundim R C 2000 Hortscience 35 904
[13] Wood, C B, Pritchard H W and Amritphale, D 2000 Seed Sci. Res. 10 135
[14] Maneesha S R 2019 J. Hortic. Sci. 14 149
[15] Furutani S C and Nagao M A 1987 Sci. Hortic-Amsterdam 32 67
[16] Zanotti R F, Dias D C, Barros R S, DaMatta F M and Oliveira G L 2014 Acta. Sci-Agron. 36 435
[17] Anburani A and Shakila A 2010 Acta Hort. 851 295
[18] Machala Z, Tarabová B, Sersenová D, Janda M and Hensel K 2018 J. Phys. D: Appl. Phys. 52 034002
[19] Traylor M J, Pavlovich M J, Karim S, Hait P, Sakiyama Y, Clark D S and Graves D B 2011 J. Phys. D: Appl. Phys. 44 472001
[20] Kamgang G, Herry J M, Meylheuc T, Brisset J L, Bellon-Fontaine M N, Doubla A and Naitali M 2009 Lett. Appl. Microbiol. 48 13
[21] Sukhani S, Punith N, Ekatpure A, Salunke G, Manjari M, Harsha R and Lakshminarayana R 2021 IEEE T. Plasma Sci. 49 551
[22] Zhou R, Li J, Zhou R, Zhang X and Yang S 2019 Innov. Food Sci. Emerg. 53 36
[23] Machado B, Tiwari B K, Richards K G, Abram F and Burgess C M 2021 Food Microbiol. 96 103708
[24] Peever T L and Higgins V J 1989 Plant Physiol. 90 867
[25] Thirumdas R, Kothakota A, Annapure U, Siliveru K, Blundell R, Gatt R and Valdramidis V P 2018 Trends Food Sci. Technol. 77 21
[26] Popov M A, Kochetov I V, Starikovskiy A Y and Aleksandrov N L 2018 J. Phys. D: Appl. Phys. 51 264003
[27] Shkurenkov I and Adamovich I V 2016 Plasma Sources Sci. T. 25 015021
[28] Hadanich, D Perédi J Juhász-Román M and Nagy B 2008 Acta Aliment. 37 077
[29] Zhou R, Zhou R, Prasad K, Fang Z, Speight R, Bazaka K and Ostrikov K K 2018 Green Chem. 20 5276
[30] Pauzaite G, Malakauskiene A, Nauciene Z, Zukiene R, Filatova I, Lyushkevich V and Mildaziene V 2018 Plasma Process Polym. 15 1700068
[31] Veselovsky V A and Veselova T V 2012 Russ. J. Plant Physl. 59 811
[32] Turner N J 2011 Chem. Rev. 111 4073
[33] Unuabonah E I, Adie G U, Onah L O and Adeyemi O G 2009 Chem. Eng. J. 155 567
[34] Basha S, Murthy Z V P and Jha B 2009 Chem. Eng. J. 147 226
[35] Kale R, Barwar S, Kane P and More S 2018 IJRASET 6 168
[36] Guo Q, Wang Y, Zhang H, Qu G, Wang T, Sun Q and Liang D 2017 Sci. Rep. 7 1
[37] Alboresi A, Gestin C, Leydecker M T, Bedu M, Meyer C and Truong H N 2005 Plant Cell Environ. 28 500
[38] Wang M, Meulen R, Visser K, Schaik H and Boer A 1998 Seed Sci. Res. 8 129
[39] Esterbauer H, Schaur R J and Zollner H 1991 Free Radical Bio. Med. 11 81
[40] Ighodaro O M and Akinloye O A 2018 Alex. J. Med. 54 287
[41] Barber S A, Walker J M and Vasey E H 1963 J. Agr. Food Chem. 11 204

Impact of microsecond-pulsed plasma-activated water on papaya seed germination and seedling growth

Impact of microsecond-pulsed plasma-activated water on papaya seed germination and seedling growth

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	Hanghang Chen(陈航航), Zijiang Yang(杨紫江), and Maodu Chen(陈茂笃)^†. State-to-state integral cross sections and rate constants for the N⁺(³P)+HD→NH⁺/ND⁺+D/H reaction: Accurate quantum dynamics studies[J]. 中国物理B, 2022, 31(9): 98204-098204.
[2]	蒋新晨, 马余强, 施夏清. Regulation of microtubule array inits self-organized dense active crowds[J]. 中国物理B, 2020, 29(7): 78201-078201.
[3]	沈壮志. Theoretical estimation of sonochemical yield in bubble cluster in acoustic field[J]. 中国物理B, 2020, 29(1): 14304-014304.
[4]	王玉良, 宿德志, 刘存海, 李慧. Quasi-classical trajectory study of H+LiH (v=0, 1, 2, j=0)→Li+H₂ reaction on a new global potential energy surface[J]. 中国物理B, 2019, 28(8): 83402-083402.
[5]	孙晶, 沈壮志, 莫润阳. Theoretical prediction of the yield of strong oxides under acoustic cavitation[J]. 中国物理B, 2019, 28(1): 14301-014301.
[6]	曾晖, 赵俊. Theoretical studies on a series of nitroaliphatic energetic compounds[J]. 中国物理B, 2014, 23(6): 63103-063103.
[7]	刘贵泉, 应和平. Motion of spiral tip driven by local forcing in excitable media[J]. 中国物理B, 2014, 23(5): 50502-050502.
[8]	高艳, 王海锋, 张吉东, 杨霞, 孙茂珠, 林振权. Evolution behavior of catalytically activated replication–decline in a coagulation process[J]. 中国物理B, 2013, 22(9): 96802-096802.
[9]	林振权, 叶高翔. Dynamic aggregation evolution of competitive societies of cooperative and noncooperative agents[J]. 中国物理B, 2013, 22(5): 58201-058201.
[10]	郭雅慧, 张凤昀, 马红章. Theoretical study of stereodynamics for the D'+DS(ν = 0,j = 0)→D'D+S abstraction reaction[J]. 中国物理B, 2013, 22(5): 53402-053402.
[11]	吴远刚, 林振权, 柯见洪. Kinetic evolutionary behavior of catalysis-select migration[J]. 中国物理B, 2012, 21(6): 68201-068201.
[12]	高艳, 王海锋, 林振权, 薛新英. Kinetics of catalytically activated aggregation–fragmentation process[J]. 中国物理B, 2011, 20(8): 86801-086801.
[13]	尹铭, 林振权, 柯见洪. Dynamic models of pest propagation and pest control[J]. 中国物理B, 2011, 20(8): 88201-088201.
[14]	赵娟, 罗一. Effect of reagent vibrational excitation and isotope substitution on the stereo-dynamics of the Ba + HF → BaF + H reaction[J]. 中国物理B, 2011, 20(4): 43402-043402.
[15]	王健, 孙秀冬, 骆素华, 姜永远, 孟庆鑫. Photochemical kinetics for holographic grating formation in phenanthrenequinone doped poly (methyl methacrylate) photopolymer[J]. 中国物理B, 2009, 18(10): 4326-4332.