Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy

doi:10.1088/1674-1056/ac4a6f

中国物理B ›› 2022, Vol. 31 ›› Issue (4): 44304-044304.doi: 10.1088/1674-1056/ac4a6f

Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy

Wentian Chen(陈文天)¹, Chao Tao(陶超)^1,†, Zizhong Hu(胡仔仲)², Songtao Yuan(袁松涛)^2,‡, Qinghuai Liu(刘庆淮)², and Xiaojun Liu(刘晓峻)^1,§

1 Ministry-of-Education Key Laboratory of Modern Acoustics, Department of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China;
2 Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medial University, Nanjing 210029, China

收稿日期:2021-08-20 修回日期:2021-11-25 接受日期:2022-01-12 出版日期:2022-03-16 发布日期:2022-03-29
通讯作者: Chao Tao, Songtao Yuan, Xiaojun Liu E-mail:taochao@nju.edu.cn;yuansongtao@vip.sina.com;liuxiaojun@nju.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant Nos. 12027808, 11874217, 11834008, 81900875, and 81770973) and Natural Science Foundation of Jiangsu Province, China (Grant No. BK 20181077).

Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy

Wentian Chen(陈文天)¹, Chao Tao(陶超)^1,†, Zizhong Hu(胡仔仲)², Songtao Yuan(袁松涛)^2,‡, Qinghuai Liu(刘庆淮)², and Xiaojun Liu(刘晓峻)^1,§

1 Ministry-of-Education Key Laboratory of Modern Acoustics, Department of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China;
2 Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medial University, Nanjing 210029, China

Received:2021-08-20 Revised:2021-11-25 Accepted:2022-01-12 Online:2022-03-16 Published:2022-03-29
Contact: Chao Tao, Songtao Yuan, Xiaojun Liu E-mail:taochao@nju.edu.cn;yuansongtao@vip.sina.com;liuxiaojun@nju.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant Nos. 12027808, 11874217, 11834008, 81900875, and 81770973) and Natural Science Foundation of Jiangsu Province, China (Grant No. BK 20181077).

摘要/Abstract

摘要： Photoacoustic imaging is a potential candidate for in vivo brain imaging, whereas, its imaging performance could be degraded by inhomogeneous multi-layered media, consisted of scalp and skull. In this work, we propose a low-artifact photoacoustic microscopy (LAPAM) scheme, which combines conventional acoustic-resolution photoacoustic microscopy with scanning acoustic microscopy to suppress the reflection artifacts induced by multi-layers. Based on similar propagation characteristics of photoacoustic signals and ultrasonic echoes, the ultrasonic echoes can be employed as the filters to suppress the reflection artifacts to obtain low-artifact photoacoustic images. Phantom experiment is used to validate the effectiveness of this method. Furthermore, LAPAM is applied for in-vivo imaging mouse brain without removing the scalp and the skull. Experimental results show that the proposed method successfully achieves the low-artifact brain image, which demonstrates the practical applicability of LAPAM. This work might improve the photoacoustic imaging quality in many biomedical applications which involve tissues with complex acoustic properties, such as brain imaging through scalp and skull.

关键词: photoacoustic microscopy, scanning acoustic microscopy, noninvasive, low-artifact, brain imaging

Abstract: Photoacoustic imaging is a potential candidate for in vivo brain imaging, whereas, its imaging performance could be degraded by inhomogeneous multi-layered media, consisted of scalp and skull. In this work, we propose a low-artifact photoacoustic microscopy (LAPAM) scheme, which combines conventional acoustic-resolution photoacoustic microscopy with scanning acoustic microscopy to suppress the reflection artifacts induced by multi-layers. Based on similar propagation characteristics of photoacoustic signals and ultrasonic echoes, the ultrasonic echoes can be employed as the filters to suppress the reflection artifacts to obtain low-artifact photoacoustic images. Phantom experiment is used to validate the effectiveness of this method. Furthermore, LAPAM is applied for in-vivo imaging mouse brain without removing the scalp and the skull. Experimental results show that the proposed method successfully achieves the low-artifact brain image, which demonstrates the practical applicability of LAPAM. This work might improve the photoacoustic imaging quality in many biomedical applications which involve tissues with complex acoustic properties, such as brain imaging through scalp and skull.

Key words: photoacoustic microscopy, scanning acoustic microscopy, noninvasive, low-artifact, brain imaging

中图分类号: (Thermoacoustics, high temperature acoustics, photoacoustic effect)

43.35.Ud

43.35.Sx (Acoustooptical effects, optoacoustics, acoustical visualization, Acoustical microscopy, and acoustical holography) 78.20.Pa (Photoacoustic effects) 81.70.Cv (Nondestructive testing: ultrasonic testing, photoacoustic testing)

Wentian Chen(陈文天), Chao Tao(陶超), Zizhong Hu(胡仔仲), Songtao Yuan(袁松涛), Qinghuai Liu(刘庆淮), and Xiaojun Liu(刘晓峻). Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy[J]. 中国物理B, 2022, 31(4): 44304-044304.

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Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy

Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy

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