中国物理B ›› 2018, Vol. 27 ›› Issue (5): 56802-056802.doi: 10.1088/1674-1056/27/5/056802

所属专题： TOPICAL REVIEW — Electron microscopy methods for emergent materials and life sciences

• SPECIAL TOPIC—Recent advances in thermoelectric materials and devices • 上一篇    下一篇

Computing methods for icosahedral and symmetry-mismatch reconstruction of viruses by cryo-electron microscopy

Bin Zhu(朱彬), Lingpeng Cheng(程凌鹏), Hongrong Liu(刘红荣)   

  1. 1 College of Physics and Information Science, Synergetic Innovation Center for Quantum Effects and Applications and Key Laboratory of Low-dimensional Quantum Structures, Hunan Normal University, Changsha 410081, China;
    2 Technology Center for Protein Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
  • 收稿日期:2017-11-21 修回日期:2018-03-27 出版日期:2018-05-05 发布日期:2018-05-05
  • 通讯作者: Lingpeng Cheng, Hongrong Liu E-mail:lingpengcheng@mail.tsinghua.edu.cn;hrliu@hunnu.edu.cn
  • 基金资助:
    Project supported by the National Key R&D Program of China (Grant No.2016YFA0501100),the National Natural Science Foundation of China (Grant Nos.91530321,31570742,and 31570727),and Science and Technology Planning Project of Hunan Province,China (Grant No.2017RS3033).

Computing methods for icosahedral and symmetry-mismatch reconstruction of viruses by cryo-electron microscopy

Bin Zhu(朱彬)1, Lingpeng Cheng(程凌鹏)2, Hongrong Liu(刘红荣)1   

  1. 1 College of Physics and Information Science, Synergetic Innovation Center for Quantum Effects and Applications and Key Laboratory of Low-dimensional Quantum Structures, Hunan Normal University, Changsha 410081, China;
    2 Technology Center for Protein Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
  • Received:2017-11-21 Revised:2018-03-27 Online:2018-05-05 Published:2018-05-05
  • Contact: Lingpeng Cheng, Hongrong Liu E-mail:lingpengcheng@mail.tsinghua.edu.cn;hrliu@hunnu.edu.cn
  • Supported by:
    Project supported by the National Key R&D Program of China (Grant No.2016YFA0501100),the National Natural Science Foundation of China (Grant Nos.91530321,31570742,and 31570727),and Science and Technology Planning Project of Hunan Province,China (Grant No.2017RS3033).

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摘要： Three-dimensional (3D) reconstruction of icosahedral viruses has played a crucial role in the development of cryo-electron microscopy single-particle reconstruction, with many cryo-electron microscopy techniques first established for structural studies of icosahedral viruses, owing to their high symmetry and large mass. This review summarizes the computational methods for icosahedral and symmetry-mismatch reconstruction of viruses, as well as the likely challenges and bottlenecks in virus reconstruction, such as symmetry mismatch reconstruction, contrast transformation function (CTF) correction, and particle distortion.

关键词: cryo-electron microscopy, icosahedral virus, computational method, three-dimensional reconstruction, symmetry-mismatch reconstruction

Abstract: Three-dimensional (3D) reconstruction of icosahedral viruses has played a crucial role in the development of cryo-electron microscopy single-particle reconstruction, with many cryo-electron microscopy techniques first established for structural studies of icosahedral viruses, owing to their high symmetry and large mass. This review summarizes the computational methods for icosahedral and symmetry-mismatch reconstruction of viruses, as well as the likely challenges and bottlenecks in virus reconstruction, such as symmetry mismatch reconstruction, contrast transformation function (CTF) correction, and particle distortion.

Key words: cryo-electron microscopy, icosahedral virus, computational method, three-dimensional reconstruction, symmetry-mismatch reconstruction

中图分类号:  (Transmission electron microscopy (TEM))

  • 68.37.Lp
68.37.Og (High-resolution transmission electron microscopy (HRTEM)) 02.20.-a (Group theory)