Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition

doi:10.1088/1674-1056/24/12/128201

中国物理B ›› 2015, Vol. 24 ›› Issue (12): 128201-128201.doi: 10.1088/1674-1056/24/12/128201

• SPECIAL TOPIC—8th IUPAP International Conference on Biological Physics • 上一篇下一篇

Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition

刘丽, 武烈, 曾丽, 姜秀娥

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

收稿日期:2015-09-30 修回日期:2015-10-20 出版日期:2015-12-05 发布日期:2015-12-05
通讯作者: Jiang Xiu-E E-mail:jiangxiue@ciac.ac.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant Nos. 91227114, 21322510, and 21105097), the China Postdoctoral Science Foundation (Grant No. 2013M530998), the Natural Science Foundation of Jilin Province, China (Grant No. 201215092), and the President Funds of the Chinese Academy of Sciences.

Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition

Liu Li (刘丽), Wu Lie (武烈), Zeng Li (曾丽), Jiang Xiu-E (姜秀娥)

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received:2015-09-30 Revised:2015-10-20 Online:2015-12-05 Published:2015-12-05
Contact: Jiang Xiu-E E-mail:jiangxiue@ciac.ac.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant Nos. 91227114, 21322510, and 21105097), the China Postdoctoral Science Foundation (Grant No. 2013M530998), the Natural Science Foundation of Jilin Province, China (Grant No. 201215092), and the President Funds of the Chinese Academy of Sciences.

摘要/Abstract

摘要： In this study, a lipid membrane was fabricated by fusing cardiolipin-phosphatidylcholine (CL_PC, 1:4) vesicles onto a hydrophobic surface of 1-dodecanethiol (DT) preadsorbed on a nanostructured gold film. By changing the concentration of the DT adsorption solution, we constructed a series of CL_PC-DT bilayers with different hydrophobicity to study the effects of lipid membrane characteristics on the adsorption conformation of cytochrome c (Cyt c). Electrochemical analysis showed that the formal potential is 0.24 V for Cyt c-CL_PC-DT(10), 0.2 V for Cyt c-CL_PC-DT(20), and 0.16 V for Cyt c-CL_PC-DT(40)–a gradual positive shift with the decreasing DT concentration–relative to the potential of native cyt c (0.02 V). Potential-induced surface-enhanced infrared adsorption difference spectroscopy revealed that the gradual positive shift of the formal potential of CL-bound cyt c is determined by the environment with the gradually lowered dielectric constant for the heme cofactor in CL-bound cyt c (Fe³⁺).

关键词: cytochrome c, cardiolipin, surface-enhanced infrared adsorption spectroscopy, protein-solvent interaction

Abstract: In this study, a lipid membrane was fabricated by fusing cardiolipin-phosphatidylcholine (CL_PC, 1:4) vesicles onto a hydrophobic surface of 1-dodecanethiol (DT) preadsorbed on a nanostructured gold film. By changing the concentration of the DT adsorption solution, we constructed a series of CL_PC-DT bilayers with different hydrophobicity to study the effects of lipid membrane characteristics on the adsorption conformation of cytochrome c (Cyt c). Electrochemical analysis showed that the formal potential is 0.24 V for Cyt c-CL_PC-DT(10), 0.2 V for Cyt c-CL_PC-DT(20), and 0.16 V for Cyt c-CL_PC-DT(40)–a gradual positive shift with the decreasing DT concentration–relative to the potential of native cyt c (0.02 V). Potential-induced surface-enhanced infrared adsorption difference spectroscopy revealed that the gradual positive shift of the formal potential of CL-bound cyt c is determined by the environment with the gradually lowered dielectric constant for the heme cofactor in CL-bound cyt c (Fe³⁺).

Key words: cytochrome c, cardiolipin, surface-enhanced infrared adsorption spectroscopy, protein-solvent interaction

中图分类号: (Solvent effects on reactivity)

82.20.Yn

82.39.Jn (Charge (electron, proton) transfer in biological systems) 82.45.Mp (Thin layers, films, monolayers, membranes) 82.45.Tv (Bioelectrochemistry)

刘丽, 武烈, 曾丽, 姜秀娥. Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition[J]. 中国物理B, 2015, 24(12): 128201-128201.

Liu Li (刘丽), Wu Lie (武烈), Zeng Li (曾丽), Jiang Xiu-E (姜秀娥). Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition[J]. Chin. Phys. B, 2015, 24(12): 128201-128201.

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Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition

Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition

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