中国物理B ›› 2012, Vol. 21 ›› Issue (5): 54211-054211.doi: 10.1088/1674-1056/21/5/054211

• ELECTROMAGNETISM, OPTICS, ACOUSTICS, HEAT TRANSFER, CLASSICAL MECHANICS, AND FLUID DYNAMICS • 上一篇    下一篇

Reflection-mode photoacoustic microscopy using a hollow focused ultrasound transducer for in vivo imaging of blood vessels

袁毅,杨思华   

  1. MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
  • 收稿日期:2011-10-22 修回日期:2012-04-27 出版日期:2012-04-01 发布日期:2012-04-01
  • 基金资助:
    Project supported by the National Basic Research Program of China (Grant Nos. 2010CB732602 and 2011CB910402), the Program for Changjiang Scholars and Innovative Research Team in University, China (Grant No. IRT0829), the National Natural Science Foundation of China (Grant Nos. 30870676, 81127004 and 11104087), and the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (Grant No. LYM10061).

Reflection-mode photoacoustic microscopy using a hollow focused ultrasound transducer for in vivo imaging of blood vessels

Yuan Yi(袁毅) and Yang Si-Hua(杨思华)†   

  1. MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
  • Received:2011-10-22 Revised:2012-04-27 Online:2012-04-01 Published:2012-04-01
  • Supported by:
    Project supported by the National Basic Research Program of China (Grant Nos. 2010CB732602 and 2011CB910402), the Program for Changjiang Scholars and Innovative Research Team in University, China (Grant No. IRT0829), the National Natural Science Foundation of China (Grant Nos. 30870676, 81127004 and 11104087), and the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (Grant No. LYM10061).

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摘要： A reflection-mode photoacoustic microscope using a hollow focused ultrasound transducer is developed for high-resolution in vivo imaging. A confocal structure of the laser and the ultrasound is used to improve the system resolution. The axial and lateral resolutions of the system are measured to be ～ 32 μm and ～ 58 μm, respectively. Ex vivo and in vivo modes are tested to validate the imaging capability of the photoacoustic microscope. The adjacent vein and artery can be seen clearly from the reconstructed photoacoustic images. The results demonstrate that the reflection-mode photoacoustic microscope can be used for high-resolution imaging of micro-blood vessels, which would be of great benefit for monitoring the neovascularization in tumor angiogenesis.

关键词: photoacoustic microscopy, hollow focused ultrasound transducer, blood vessels

Abstract: A reflection-mode photoacoustic microscope using a hollow focused ultrasound transducer is developed for high-resolution in vivo imaging. A confocal structure of the laser and the ultrasound is used to improve the system resolution. The axial and lateral resolutions of the system are measured to be ～ 32 μm and ～ 58 μm, respectively. Ex vivo and in vivo modes are tested to validate the imaging capability of the photoacoustic microscope. The adjacent vein and artery can be seen clearly from the reconstructed photoacoustic images. The results demonstrate that the reflection-mode photoacoustic microscope can be used for high-resolution imaging of micro-blood vessels, which would be of great benefit for monitoring the neovascularization in tumor angiogenesis.

Key words: photoacoustic microscopy, hollow focused ultrasound transducer, blood vessels

中图分类号:  (Laser applications)

  • 42.62.-b