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Influence of fluorescence time characteristics on the spatial resolution of CW-stimulated emission depletion microscopy |
| Haiyun Qin(秦海芸)1, Wei Zhao(赵伟)1, Chen Zhang(张琛)1, Yong Liu(刘勇)2, Guiren Wang(王归仁)3, Kaige Wang(王凯歌)1 |
1 Institute of Photonics and Photon-Technology, Northwest University, Xi'an 710069, China;
2 School of Electronics and Information Engineering, Shanghai University of Electric Power, Shanghai 200090, China;
3 Mechanical Engineering Department & Biomedical Engineering Program, University of South Carolina, Columbia, SC 29208, USA |
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Abstract As one of the most important realizations of stimulated emission depletion (STED) microscopy, the continuous-wave (CW) STED system, constructed by using CW lasers as the excitation and STED beams, has been investigated and developed for nearly a decade. However, a theoretical model of the suppression factors in CW STED has not been well established. In this investigation, the factors that affect the spatial resolution of a CW STED system are theoretically and numerically studied. The full-width-at-half-maximum (FWHM) of a CW STED with a doughnut-shaped STED beam is also reanalyzed. It is found that the suppression function is dominated by the ratio of the local STED and excitation beam intensities. In addition, the FWHM is highly sensitive to both the fluorescence rate (inverse of fluoresce lifetime) and the quenching rate, but insensitive to the rate of vibrational relaxation. For comparison, the suppression function in picosecond STED is only determined by the distribution of the STED beam intensity scaled with the saturation intensity. Our model is highly consistent with published experimental data for evaluating the spatial resolution. This investigation is important in guiding the development of new CW STED systems.
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Received: 13 November 2017
Revised: 13 December 2017
Accepted manuscript online:
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PACS:
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78.45.+h
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(Stimulated emission)
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78.55.-m
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(Photoluminescence, properties and materials)
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| Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 11672229 and 61378083), International Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2011DFA12220), Major Research Plan of the National Natural Science Foundation of China (Grant No. 91123030), Natural Science Foundation of Shaanxi Province of China (Grant Nos. 2010JS110 and 2013SZS03-Z01), Natural Science Basic Research Program of Shaanxi Province - Major Basic Research Project, China (Grant No. 2016ZDJC-15), Young Scientist Fund of the National Natural Science Foundation of China (Grant No. 11504294), and the Youth Talent Plan of the Natural Science Foundation of Shaanxi Province of China (Grant No. 2016JQ103).{These authors contributed equally to this work. |
Corresponding Authors:
Kaige Wang
E-mail: wangkg@nwu.edu.cn
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Cite this article:
Haiyun Qin(秦海芸), Wei Zhao(赵伟), Chen Zhang(张琛), Yong Liu(刘勇), Guiren Wang(王归仁), Kaige Wang(王凯歌) Influence of fluorescence time characteristics on the spatial resolution of CW-stimulated emission depletion microscopy 2018 Chin. Phys. B 27 037803
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