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Abstract With recent breakthroughs in camera and image processing technologies single-particle electron cryo-microscopy (CryoEM) has suddenly gained the attention of structural biologists as a powerful tool able to solve the atomic structures of biological complexes and assemblies. Compared with x-ray crystallography, CryoEM can be applied to partially flexible structures in solution and without the necessity of crystallization, which is especially important for large complexes and assemblies. This review briefly explains several key bottlenecks for atomic resolution CryoEM, and describes the corresponding solutions for these bottlenecks based on the recent technical advancements. The review also aims to provide an overview about the technical differences between its applications in biology and those in material science.
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Received: 13 July 2015
Revised: 31 August 2015
Accepted manuscript online:
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PACS:
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87.64.Ee
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(Electron microscopy)
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87.80.-y
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(Biophysical techniques (research methods))
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87.15.B-
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(Structure of biomolecules)
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| Fund: Project supported by Tsinghua-Peking Joint Center for Life Sciences, China |
Corresponding Authors:
Xueming Li
E-mail: lixueming@tsinghua.edu.cn
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Cite this article:
Xueming Li(李雪明) Recent technical advancements enabled atomic resolution CryoEM 2016 Chin. Phys. B 25 018710
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