Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro

doi:10.1088/1674-1056/20/2/024301

Abstract Microbubbles promise to enhance the efficiency of ultrasound-mediated drug delivery and gene therapy by taking advantage of artificial cavitation nuclei. The purpose of this study is to examine the ultrasound-induced hemolysis in the application of drug delivery in the presence of microbubbles. To achieve this goal, human red blood cells mixed with microbubbles were exposed to 1-MHz pulsed ultrasound. The hemolysis level was measured by a flow cytometry, and the cavitation dose was detected by a passive cavitation detecting system. The results demonstrate that larger cavitation dose would be generated with the increase of acoustic pressure, which might give rise to the enhancement of hemolysis. Besides the experimental observations, the acoustic pressure dependence of the radial oscillation of microbubble was theoretically estimated. The comparison between the experimental and calculation results indicates that the hemolysis should be highly correlated to the acoustic cavitation.

Keywords: acoustic cavitation passive cavitation detection microbubbles hemolysis

Received: 17 June 2010
Revised: 12 July 2010
Accepted manuscript online:

PACS:	43.25.+y	(Nonlinear acoustics)
	43.35.+d	(Ultrasonics, quantum acoustics, and physical effects of sound)
	43.80.+p	(Bioacoustics)

Fund: Project supported by the National Basic Research Program of China (Grant No. 2010CB732600), the National Natural Science Foundation of China (Grant Nos. 10774071, 10974093, 10974098, and 30672014), the Natural Science Foundation of Jiangsu Province of China (Grant NO. BE2010768), and the Fund of the State Key Lab of Acoustics.

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Zhang Chun-Bing(张春兵), Liu Zheng(刘政), Guo Xia-sheng(郭霞生), and Zhang Dong(章东) Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro 2011 Chin. Phys. B 20 024301

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Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro

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