Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

doi:10.1088/1674-1056/19/4/049501

Abstract This paper first demonstrates second-harmonic generation (SHG) in the intact cell nucleus, which acts as an optical indicator of DNA malignancy in prostate glandular epithelial cells. Within a scanning region of $2.7~\mu$m$\times 2.7~\mu$m in cell nuclei, SHG signals produced from benign prostatic hyperplasia (BPH) and prostate carcinoma (PC) tissues (mouse model C57BL/6) have been investigated. Statistical analyses ($t$ test) of a total of 405 measurements (204 nuclei from BPH and 201 nuclei from PC) show that SHG signals from BPH and PC have a distinct difference ($p < 0.05)$, suggesting a potential optical method of revealing very early malignancy in prostate glandular epithelial cells based upon induced biochemical and/or biophysical modifications in DNA.

Keywords: nonlinear microscopy multiharmonic generation diagnostic applications of nonlinear optics

Received: 02 January 2009
Revised: 10 October 2009
Accepted manuscript online:

PACS:	87.14.G-	(Nucleic acids)
	42.65.Ky	(Frequency conversion; harmonic generation, including higher-order harmonic generation)
	87.19.X-	(Diseases)
	87.15.N-	(Properties of solutions of macromolecules)
	87.15.M-	(Spectra of biomolecules)

Fund: Project supported by the National Natural Science Foundation of China (Grant No.~30470495), the Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Normal University.

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Zhuang Zheng-Fei(庄正飞), Liu Han-Ping(刘汉平), Guo Zhou-Yi(郭周义), Zhuo Shuang-Mu(卓双木), Yu Bi-Ying(喻碧英), and Deng Xiao-Yuan(邓小元) Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells 2010 Chin. Phys. B 19 049501

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Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

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