Investigation of europium(Ⅲ)-doped ZnS for immunoassay

doi:10.1088/1674-1056/27/2/027803

Abstract Biofunctional europium (Ⅲ)-doped ZnS (ZnS:Eu) nanocrystals are prepared by a sol-gel method. The characteristic luminescence of ZnS:Eu is used as a probe signal to realize sensitive immunoassay. The luminescence intensity of the Eu³⁺ in the ZnS matrix shows strong concentration dependence, and the optimal doping concentration is 4%. However, the emission wavelengths of the ZnS:Eu nanocrystals are not dependent on doping concentration nor the temperature (from 100 K to 300 K). Our results show that these features allow for reliable immunoassay. Human immunoglobulin, used as a target analyte, is captured by antibody modified ZnS:Eu probe and is finally enriched on gold substrate for detection. High specificity of the assay is demonstrated by control experiments. The linear detection range is 10 nM-800 nM, and the detection limit is about 9.6 nM.

Keywords: ZnS:Eu optical properties immunoassay

Received: 20 July 2017
Revised: 18 October 2017
Accepted manuscript online:

PACS:	78.55.-m	(Photoluminescence, properties and materials)
	68.35.bg	(Semiconductors)
	78.55.Et	(II-VI semiconductors)

Fund: Project supported by the National Natural Science Foundation of China (Grant No. 61205193), the Project of Science and Technology of Jilin Province, China (Grant No. 20140520107JH), the Technology Foundation of Jilin Provincial Department of Human Resources and Social Security, China (Grant No. RL201306), and the Science Foundation for Young Scientists of Changchun University of Science and Technology, China (Grant No. XQNJJ-2015-03).

Corresponding Authors: Xue-Ying Chu
E-mail: chuxy608@163.com

About author: 78.55.-m; 68.35.bg; 78.55.Et

Cite this article:

Chao-Fan Zhu(朱超凡), Xue Sha(沙雪), Xue-Ying Chu(楚学影), Jin-Hua Li(李金华), Ming-Ze Xu(徐铭泽), Fang-Jun Jin(金芳军), Zhi-Kun Xu(徐志堃) Investigation of europium(Ⅲ)-doped ZnS for immunoassay 2018 Chin. Phys. B 27 027803

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Investigation of europium(Ⅲ)-doped ZnS for immunoassay

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